Powder: -20°C for 3 years | In solvent: -80°C for 1 year
BRD0209 是一种有效、选择性的 GSK3α/β 抑制剂
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 975 | 现货 | ||
5 mg | ¥ 2,490 | 现货 | ||
10 mg | ¥ 3,730 | 现货 | ||
25 mg | ¥ 5,970 | 现货 | ||
50 mg | ¥ 8,420 | 现货 | ||
100 mg | ¥ 11,300 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 2,450 | 现货 |
产品描述 | BRD0209 is a highly selective and potent GSK3 inhibitor. |
靶点活性 | GSK-3β:5 nM, GSK-3α:19 nM |
体外活性 | BRD0209 showed concentration-dependent inhibition of Tau with cellular EC50s of 1.76 μM, respectively, in SH-SY5Y cells using ELISA. Treatment of SH-SY5Y cells with BRD0209 (10 μM) significantly inhibited GSK3 kinase activity within 30 min, as seen in the decrease in phospho-CRMP2 and phospho-β-catenin. BRD0209 is also a reversible ATP-competitive inhibitor with fast-off kinetics (Ki = 4.2 nM, respectively).BRD0209 treatment caused a dramatic decrease in autophosphorylation at Tyr279 (pGSK3α) and Tyr216 (pGSK3β) with longer exposure (24 h) [1]. |
别名 | BRD-0209, BRD 0209 |
分子量 | 347.45 |
分子式 | C22H25N3O |
CAS No. | 1597439-87-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 3.47 mg/mL (10 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.8781 mL | 14.3906 mL | 28.7811 mL | 71.9528 mL |
5 mM | 0.5756 mL | 2.8781 mL | 5.7562 mL | 14.3906 mL | |
10 mM | 0.2878 mL | 1.4391 mL | 2.8781 mL | 7.1953 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
BRD0209 1597439-87-6 PI3K/Akt/mTOR signaling Stem Cells GSK-3 BRD-0209 BRD 0209 Inhibitor inhibitor inhibit