Powder: -20°C for 3 years | In solvent: -80°C for 1 year
BOT-64 是一种 IKK-2 抑制剂,靶向激酶激活环结构域中的 Ser177 和/或 Ser181 残基。BOT-64 是一种可渗透细胞的苯并恶硫醇化合物。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 786 | 现货 | ||
5 mg | ¥ 1,990 | 现货 | ||
10 mg | ¥ 3,160 | 现货 | ||
25 mg | ¥ 5,990 | 现货 | ||
50 mg | ¥ 7,910 | 现货 | ||
100 mg | ¥ 10,800 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,790 | 现货 |
产品描述 | BOT-64 is an IKK-2 inhibitor which targets the Ser177 and/or Ser181 residues in the kinase's activation loop domain.BOT-64 is a cell-permeable benzoxathiole compound |
体外活性 | BOT-64 inhibits IKKbeta-mediated IkappaBalpha phosphorylation in LPS-activated macrophages, resulting in sequential prevention of downstream events, including proteolytic degradation of IkappaBalpha, DNA binding ability, and transcriptional activity of NF-kappaB.?BOT-64 inhibits LPS-inducible IKKbeta activity in the cells and catalytic activity of highly purified IKKbeta.?Moreover, the effect of BOT-64 on cell-free IKKbeta was abolished by substitution of Ser-177 and Ser-181 residues in the activation loop of IKKbeta to glutamic acid residues, indicating a direct interaction site of benzoxathiole.?BOT-64 attenuates NF-kappaB-regulated expression of inflammatory genes such as inducible nitric-oxide synthase, cyclooxygenase-2, tumor necrosis factor-alpha, interleukin (IL)-1beta, and IL-6 in LPS-activated or expression vector IKKbeta-transfected macrophages. |
分子量 | 273.35 |
分子式 | C15H15NO2S |
CAS No. | 113760-29-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 30 mg/mL (109.75 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.6583 mL | 18.2916 mL | 36.5831 mL | 91.4578 mL |
5 mM | 0.7317 mL | 3.6583 mL | 7.3166 mL | 18.2916 mL | |
10 mM | 0.3658 mL | 1.8292 mL | 3.6583 mL | 9.1458 mL | |
20 mM | 0.1829 mL | 0.9146 mL | 1.8292 mL | 4.5729 mL | |
50 mM | 0.0732 mL | 0.3658 mL | 0.7317 mL | 1.8292 mL | |
100 mM | 0.0366 mL | 0.1829 mL | 0.3658 mL | 0.9146 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
BOT-64 113760-29-5 NF-Κb IκB/IKK IL-6 I kappa B kinase inhibit inflammatory BOT64 NF-κb RAW 264.7 IKK IκB kinase Inhibitor BOT 64 inhibitor