Powder: -20°C for 3 years | In solvent: -80°C for 1 year
BG45是一个 HDAC I 型抑制剂,选择性抑制 HDAC3,IC50为289 nM。它抑制 HDAC1/2/6的效力大大降低,IC50分别为2、2.2和<20μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 297 | 现货 | ||
5 mg | ¥ 493 | 现货 | ||
10 mg | ¥ 688 | 现货 | ||
25 mg | ¥ 1,320 | 现货 | ||
50 mg | ¥ 2,320 | 现货 | ||
100 mg | ¥ 3,430 | 现货 | ||
200 mg | ¥ 4,750 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 483 | 现货 |
产品描述 | BG45 is an HDAC I type inhibitor with IC50 of 289 nM, 2.0 μM, 2.2 μM and >20 μM for HDAC3/1/2/6 in cell-free assays, respectively. |
靶点活性 | HDAC1:2 μM, HDAC3:289 nM, HDAC2:2.2 μM |
体外活性 | BG45 causes significant cell growth inhibition and induces caspase-dependent apoptosis in a series of MM cells. In RPMI8226 cells, BG45 synergistically enhances bortezomib-induced cytotoxicity. [1] |
体内活性 | In mice bearing MM.1S xenograft of human MM, BG45 (50 mg/kg, i.p.) significantly inhibits MM tumor growth. When in combination with bortezomib, BG45 (50 mg/kg, i.p.) enhances bortezomib-induced cytotoxicity. [1] |
激酶实验 | Kinase assays: Initial rate constants are measured at 21 °C in the presence of 100 mM HEPES (pH 7.5), 0.3 mg/mL poly(Glu-Tyr) peptide substrate, 10 mM MgCl2, 1 mg/mL bovine serum albumin, 5% DMSO, 20 nM MET-KD and various concentrations of ATP and SGX523. Total reaction volumes (20 μL) are quenched with 20 μL Kinase-Glo detection buffer. Luminescence is detected in a plate-reading luminometer and the results are analyzed by nonlinear regression. |
细胞实验 | The growth inhibitory effects of BG45 in MM cell lines are assessed by measuring 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide (MTT) dye absorbance. To measure proliferation of MM cells, the rate of DNA synthesis is measured by [3H]thymidine uptake.(Only for Reference) |
分子量 | 214.22 |
分子式 | C11H10N4O |
CAS No. | 926259-99-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 2 mg/mL (9.33 mM), Heating is recommended.
DMSO: 39 mg/mL (182.1 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 4.6681 mL | 23.3405 mL | 46.681 mL | 116.7025 mL |
5 mM | 0.9336 mL | 4.6681 mL | 9.3362 mL | 23.3405 mL | |
DMSO | 10 mM | 0.4668 mL | 2.334 mL | 4.6681 mL | 11.6702 mL |
20 mM | 0.2334 mL | 1.167 mL | 2.334 mL | 5.8351 mL | |
50 mM | 0.0934 mL | 0.4668 mL | 0.9336 mL | 2.334 mL | |
100 mM | 0.0467 mL | 0.2334 mL | 0.4668 mL | 1.167 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
BG45 926259-99-6 Apoptosis Chromatin/Epigenetic DNA Damage/DNA Repair HDAC Inhibitor inhibit Histone deacetylases BG 45 BG-45 inhibitor