Powder: -20°C for 3 years | In solvent: -80°C for 1 year
BD750 是免疫抑制剂和JAK3/STAT5的抑制剂,可抑制 IL-2 诱导的、JAK3/STAT5 依赖的 T 细胞增殖,对小鼠和人 T 细胞增殖的IC50值分别为 1.5 和 1.1 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 218 | 现货 | ||
5 mg | ¥ 497 | 现货 | ||
10 mg | ¥ 828 | 现货 | ||
25 mg | ¥ 1,490 | 现货 | ||
50 mg | ¥ 2,480 | 现货 | ||
100 mg | ¥ 3,680 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 516 | 现货 |
产品描述 | BD750 is an effective immunosuppressant and a JAK3/STAT5 inhibitor, inhibits IL-2-induced JAK3/STAT5-dependent T cell proliferation(IC50 of 1.5 μM and 1.1 μM in mouse and human T cells, respectively) |
体外活性 | Following BD750 treatment, LPS-induced maturation of DC, allogeneic T cell proliferation, Th1 and Th17 cell functional differentiation, the STAT5 and AKT activation were determined.?The effect of tolDC loaded with antigen peptide on the development and severity of EAE and their splenic Th1 and Th17 cell responses were determined.?In comparison with LPS-induced mature DC (mDC), BD750 treatment induced tolDC with lower expression levels of costimulatory molecules and pro-inflammatory cytokines and lower levels of STAT5 phosphorylation.?TolDC inhibited allogeneic T cell proliferation and reduced Th1 and Th17 responses[1]. |
体内活性 | Adoptive transfer of tolDC loaded with MOG35-55 inhibited the development and severity of EAE in mice, accompanied by reduced numbers of inflammatory infiltrates and decreased levels of demyelination in the spinal cord tissues of mice.?In addition, treatment with tolDC loaded with antigen peptide also significantly reduced the frequency of splenic Th1 and Th17 cells in EAE mice.?The effects of tolDC were similar to that of the JAK/STAT inhibitor, CP690550-treated DC[1]. |
动物实验 | Female C57BL/6 mice (10 wks old, 19-21 g).Dosage with 20 μM, and administration:IV, treated DC on d 7, 11 and 15 post the first PTX injection (dpi 7, 11 and 15)[1]. |
分子量 | 271.34 |
分子式 | C14H13N3OS |
CAS No. | 892686-59-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 27.5 mg/mL (101.35 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.6854 mL | 18.4271 mL | 36.8541 mL | 92.1353 mL |
5 mM | 0.7371 mL | 3.6854 mL | 7.3708 mL | 18.4271 mL | |
10 mM | 0.3685 mL | 1.8427 mL | 3.6854 mL | 9.2135 mL | |
20 mM | 0.1843 mL | 0.9214 mL | 1.8427 mL | 4.6068 mL | |
50 mM | 0.0737 mL | 0.3685 mL | 0.7371 mL | 1.8427 mL | |
100 mM | 0.0369 mL | 0.1843 mL | 0.3685 mL | 0.9214 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
BD750 892686-59-8 Angiogenesis Chromatin/Epigenetic JAK/STAT signaling Stem Cells JAK STAT Immunosuppressant BD-750 BD 750 cell Janus kinase inhibit Inhibitor inhibitor