Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Azithromycin (CP 62993) 是一种常用于细菌感染研究的大环内酯抗生素。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 680 | 现货 | ||
100 mg | ¥ 1,062 | 现货 | ||
500 mg | ¥ 3,211 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 748 | 现货 |
产品描述 | Azithromycin (CP 62993) is an antibiotic by inhibiting protein synthesis, used for the treatment of bacterial infections. |
体外活性 | Azithromycin reduces about 40% of IL-8 mRNA and protein expression in cystic fibrosis (CF) cells reaching the levels of non-CF cells. Azithromycin results in 50% and 70% reduction of NF-kappaB and AP-1 DNA binding, respectively, leading to levels of non-CF cells. Azithromycin significantly enhances the intensity of a co-stimulatory molecule, CD80, on DCs but not CD86 and CD40 in dendritic cells (DCs). Azithromycin significantly increases the production of IL-10 and Clarithromycin (CAM) significantly inhibits the production of IL-6 by DCs. Azithromycin increases IL-10 and CAM decreases IL-2 productions significantly, when naive T cells derived from spleen are co-cultured with DCs treated in advance with LPS and these macrolides.Azithromycin selectively inhibits fluid-phase endocytosis of horseradish peroxidase and lucifer yellow in J774 mouse macrophages. Azithromycin delays sequestration of receptor-bound transferrin and peroxidase-anti-peroxidase immune complexes into cell-surface endocytic pits and vesicles. Azithromycin down-regulates cell surface transferrin receptors, but not Fc gamma receptors, by causing a major delay in the accessibility of internalized transferrin receptors to the recycling route, without slowing down subsequent efflux, resulting in redistribution of the surface pool to an intracellular pool.Azithromycin inserts into the DOPC lipid bilayer, so as to decrease its cohesion and to facilitate the merging of DPPC into the DOPC fluid matrix. |
细胞实验 | THP-1 cells (106 cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm. |
别名 | 阿奇霉素, Zithromax, CP 62993, XZ-450 |
分子量 | 748.98 |
分子式 | C38H72N2O12 |
CAS No. | 83905-01-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 74.9 mg/mL (100 mM)
Ethanol: 74.9 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 1.3351 mL | 6.6757 mL | 13.3515 mL | 33.3787 mL |
5 mM | 0.267 mL | 1.3351 mL | 2.6703 mL | 6.6757 mL | |
10 mM | 0.1335 mL | 0.6676 mL | 1.3351 mL | 3.3379 mL | |
20 mM | 0.0668 mL | 0.3338 mL | 0.6676 mL | 1.6689 mL | |
50 mM | 0.0267 mL | 0.1335 mL | 0.267 mL | 0.6676 mL | |
100 mM | 0.0134 mL | 0.0668 mL | 0.1335 mL | 0.3338 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Azithromycin 83905-01-5 Autophagy Microbiology/Virology Others Antibacterial Antibiotic CP62993 inhibit 阿奇霉素 XZ 450 CP-62993 Bacterial XZ450 Zithromax CP 62993 XZ-450 Inhibitor inhibitor