Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Auranofin (SKF-39162) 是硫氧还蛋白还原酶抑制剂,IC50为0.2 μM。它也是一种抗风湿剂,用于治疗类风湿性关节炎,改善关节炎症状。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 179 | 现货 | ||
5 mg | ¥ 385 | 现货 | ||
10 mg | ¥ 622 | 现货 | ||
25 mg | ¥ 1,170 | 现货 | ||
50 mg | ¥ 1,890 | 现货 | ||
100 mg | ¥ 2,890 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 448 | 现货 |
产品描述 | Auranofin (SKF-39162) is an antirheumatic agent, is used to treat rheumatoid arthritis, improves arthritis symptoms including painful or tender and swollen joints and morning stiffness. |
靶点活性 | thioredoxin reductase (TrxR):0.2 μM |
体外活性 | Auranofin is a drug that is approved for the treatment of rheumatoid arthritis but is being investigated for potential therapeutic application in many other diseases including cancer, neurodegenerative disorders. Auranofin induces apoptosis in cells through a Bax/Bak-dependent mechanism associated with selective disruption of mitochondrial redox homeostasis in conjunction with oxidation of Prx3[1]. Auranofin inhibits proliferation and survival of SKOV3 cells in a dose- and time-dependent manner. Auranofin treatment activates the pro-apoptotic caspase-3, increases protein levels of apoptosis-inducing proteins Bax and Bim and reduces the expression of the anti-apoptotic mediator Bcl-2 in SKOV3 cells[2]. Auranofin is a lipophilic gold compound with anti-inflammatory and immunosuppressive properties. Auranofin inhibits the cell growth and induction of mitochondrial apoptosis in PC3 human prostate cancer cells. Treatment with auranofin significantly inhibits cell viability with an IC50 value of 2.5 μM after 24 h[3]. |
体内活性 | Prophylactic treatment of adjuvant-induced arthritis rats with auranofin results in a slight reduction in paw edema, a complete normalization of the depressed IL-2 production, and a reduction of the elevated IL-1 production, without effect on the depressed IL-3 production[4]. |
细胞实验 | Auranofin is dissolved in DMSO. Cells are treated with auranofin (0, 50, 100, 200 and 400 nM) for 72 h for the dose-dependent response assay and 100 nM of auranofin is added into the wells for 0, 24, 72 and 120 h for the time-dependent response assay. Control cultures are treated with DMSO. Cell viability is measured by the MTT assay[2]. |
别名 | 醋硫葡金, SKF-39162 |
分子量 | 678.49 |
分子式 | C20H34AuO9PS |
CAS No. | 34031-32-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 22.5 mg/mL (33.16 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.4739 mL | 7.3693 mL | 14.7386 mL | 36.8465 mL |
5 mM | 0.2948 mL | 1.4739 mL | 2.9477 mL | 7.3693 mL | |
10 mM | 0.1474 mL | 0.7369 mL | 1.4739 mL | 3.6847 mL | |
20 mM | 0.0737 mL | 0.3685 mL | 0.7369 mL | 1.8423 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Auranofin 34031-32-8 Microbiology/Virology Others SARS-CoV Antibacterial SARS coronavirus 醋硫葡金 inhibit Bacterial SKF-39162 Inhibitor SKF39162 SKF 39162 inhibitor