Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Artemisinin (Qinghaosu) 是一种倍半萜烯内酯,是从青蒿植物的地上部分分离的一种抗疟疾药物。它有神经保护和抗癌作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 mg | ¥ 100 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 140 | 现货 |
产品描述 | Artemisinin (Qinghaosu) is an ancient Chinese herbal therapy for malarial fevers which has been recently found to have potent activity against many forms of malarial organisms, including chloroquine-resistant Plasmodium falciparum. |
体内活性 | Artemisinin(50、100或200 mg/kg/天,口服)可预防异氟醚引起的T迷宫测试中观察到的工作记忆损伤。Artemisinin 能增强接触异氟醚的大鼠的空间导航和记忆能力。与单纯接触异氟醚的大鼠相比,接受Artemisinin治疗的大鼠表现出明显更好的性能[4]。 |
激酶实验 | Briefly, PC12 cells are lysed in lysis buffer and centrifuged at 12,500×g for 5 min 15 mL of cell lysate is incubated with 50 mL of 2X substrate working solution at room temperature for 30 min in 96-well plates. The fluorescence intensity is then determined by Infinite M200 PRO Multimode Microplate at an excitation wavelength of 490 nm and emission at 520 nm. The fluorescence intensity of each sample is normalized to the protein concentration of sample. All values of % caspase 3/7 activities are normalized to the control group. |
细胞实验 | Artemisinin is diluted in 0.01% DMSO. For this purpose, cells are cultivated in 96-well plates in DMEM, supplemented with insulin. The artemisinin, Dox, and DDP are added to media at different concentrations and the cells are cultivated for either 24 or 48 h. For this purpose artemisinin is diluted in 0.01% DMSO in media. After this time, 10 μL of the MTT dye solution (5 mg/mL in phosphate buffer saline) is added to the cells; the cells are incubated at the same conditions for 3 h. After centrifugation (1500 rpm, 5 min) the supernatant is removed. 100 μL of dimethyl sulfoxide is added to each well, to dissolve formazan. The absorption is measured, using a multi-well spectrophotometer at a wavelength of 540 nm. |
别名 | Qinghaosu,Artemisinine, Qinghaosu, NSC 369397, 青蒿素, Artemisinine |
分子量 | 282.33 |
分子式 | C15H22O5 |
CAS No. | 63968-64-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 28.2 mg/mL (100 mM)
Ethanol: 21.2 mg/mL (75 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 3.542 mL | 17.7098 mL | 35.4195 mL | 88.5489 mL |
5 mM | 0.7084 mL | 3.542 mL | 7.0839 mL | 17.7098 mL | |
10 mM | 0.3542 mL | 1.771 mL | 3.542 mL | 8.8549 mL | |
20 mM | 0.1771 mL | 0.8855 mL | 1.771 mL | 4.4274 mL | |
50 mM | 0.0708 mL | 0.3542 mL | 0.7084 mL | 1.771 mL | |
DMSO | 100 mM | 0.0354 mL | 0.1771 mL | 0.3542 mL | 0.8855 mL |
中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
黄花蒿 | Artemisia annua Linn. | 寒 | 苦 |
方剂名称 | 处方组成 | 剂型 | 处方来源 |
人中白丸 | 人中白,羚羊角,生地黄,当归,黄花蒿,银柴胡,鳖甲,阿胶,白术,白芍,熟地黄 | 丸剂 | 《嵩崖尊生》卷十一。 |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Artemisinin 63968-64-9 Apoptosis Cytoskeletal Signaling Microbiology/Virology PI3K/Akt/mTOR signaling Proteases/Proteasome HCV Protease Ferroptosis Akt Parasite Qinghaosu,Artemisinine Qinghaosu Hepatitis C virus NSC-369397 Inhibitor inhibit Protein kinase B HCV NSC 369397 PKB 青蒿素 NSC369397 Artemisinine inhibitor