首页工具

购物车

Anisomycin

产品编号 T6758 CAS 22862-76-6

别名: 茴香霉素, Flagecidin, NSC 76712, Wuningmeisu C

Anisomycin (NSC-76712) 是一种从各种链霉菌属中分离出来的抗生素。它通过抑制肽基转移酶或 80S 核糖体系统来干扰蛋白质和 DNA 合成。

TargetMol的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

Anisomycin, CAS 22862-76-6

规格	价格/CNY	货期
10 mg	￥ 415	现货
25 mg	￥ 582	现货
50 mg	￥ 828	现货
100 mg	￥ 1,467	现货
200 mg	￥ 2,050	现货
1 mL * 10 mM (in DMSO)	￥ 410	现货

大包装超大折扣，点击咨询

选择批次

纯度: 99.81%

纯度: 99.66%

纯度: 99.11%

纯度: 98.87%

更多批次查询请联系客服

天然产物信息

生物活性

化学信息

存储 & 溶解度

参考文献

相关产品

植物来源	天然产物 > 其他（植物来源） > 其他

产品描述	Anisomycin (NSC-76712) is an antibiotic isolated from various Streptomyces species. It interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system.
体外活性	Anisomycin (3 μM) decreases protein synthesis in MDA16 and MDA-MB-468 cells, and reduces colony formation by MDA-MB-468 cells. Anisomycin causes an increase in the number of apoptotic cells in MDA-MB-468 cultures, but not in MDA16 cultures. Anisomycin actives JNK phosphorylation in MDA-MB-468 cells.[2] In U251 and U87 cells, anisomycin?(0.01-8 μM) inhibits the cell growth in time- and concentration-dependent manners with the IC50 (48 h) values of 0.233 and 0.192 μmol/L, respectively. Anisomycin?(4 μM) causes 21.5% and 25.3% of apoptosis proportion in U251 and U87 cells, respectively, and activates p38 MAPK and?JNK, while inactivated ERK1/2. Anisomycin?(4 μM) reduces the level of PP2A/C subunit in a time-dependent manner in U251 and U87 cells.[3] Anisomycin inhibits EAC cell proliferation in concentration-dependent manner.[4]
体内活性	Peritumoral administration of anisomycin (5 mg/kg) significantly suppresses Ehrlich ascites carcinoma (EAC) growth resulting in the survival of approximately 60% of the mice 90 days after EAC inoculation.[4]
激酶实验	JNK phosphorylation: 500,000 cells/well are seeded in 6-well plates and incubated overnight. Cells are then incubated for 1 h with test compounds or DMSO as vehicle control (?nal concentration 1% v/v). Puromycin is added (?nal concentration of 18 μM) and cells incubated for a further 10 min to label nascent polypeptide chains. Background labelling is determined by incubating cells without puromycin. Cells are then washed in HBSS, harvested by scraping and centrifuged (300 g, 5 min). Cells are resuspended in 0.5 mL 50 mM DTT containing phosphatase inhibitors and incubated at 95℃ for 10 min. Samples are then snap frozen in liquid nitrogen and stored at -20℃ until blotted. Samples (20–30 μg protein/sample) are blotted onto a PVDF membrane. The membrane is blocked and incubated with anti-phospho-Thr183/Tyr185-JNK antibody overnight at 4℃. Secondary antibodies are used to label the primary antibody and detected using an infrared scanner. The intensity of the ?uorescence signal for anti-phospho-JNK antibody is background corrected and normalized for loading.
细胞实验	For the assay, EAC cells are plated in 96-well plates at a density of 10,000 cells/well/200 μL of medium. The cells are treated with the different concentrations of anisomycin for 48 h. Adriamycin (500 ng/mL) is used as a positive control. 0.5 mg/mL of MTT is added to each well. 4 h later, the formazan product of MTT reduction is dissolved in DMSO, and absorbance is measured at 570 nm using a Model 680 microplate reader.(Only for Reference)

别名	茴香霉素, Flagecidin, NSC 76712, Wuningmeisu C
化合物与蛋白结合的复合物	Co-crystal Structure of Anisomycin Bound to the 50S Ribosomal Subunit
分子量	265.3
分子式	C14H19NO4
CAS No.	22862-76-6

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

Ethanol: 13.3 mg/mL (50 mM)

DMSO: 26.5 mg/mL (100 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	3.7693 mL	18.8466 mL	37.6932 mL	94.2329 mL
	5 mM	0.7539 mL	3.7693 mL	7.5386 mL	18.8466 mL
	10 mM	0.3769 mL	1.8847 mL	3.7693 mL	9.4233 mL
	20 mM	0.1885 mL	0.9423 mL	1.8847 mL	4.7116 mL
	50 mM	0.0754 mL	0.3769 mL	0.7539 mL	1.8847 mL
DMSO	100 mM	0.0377 mL	0.1885 mL	0.3769 mL	0.9423 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

质量

浓度

容积

分子量

浓度 (起始)

容积 (起始)

浓度 (终)

容积 (终)

溶液体积

质量

配液浓度

参考文献

1. Iordanov MS, et al. Mol Cell Biol. 1997, 17(6), 3373-3381. 2. Monaqhan D, et al. Biochem Biophys Res Commun. 2014, 443(2), 761-767. 3. Li JY, et al. Acta Pharmacol Sin. 2012, 33(7), 935-940. 4. You P, et al. Oncol Rep. 2013, 29(6), 2227-2236. 5. Chen L, Zhou X, Kong X, et al. The Prognostic Significance of Anisomycin-Activated Phospho-c-Jun NH2-Terminal Kinase (p-JNK) in Predicting Breast Cancer Patients’ Survival Time[J]. Frontiers in Cell and Developmental Biology. 2021, 9: 470. 6. Liu Y J, Chang Y J, Kuo Y T, et al. Targeting β-tubulin/CCT-β complex induces apoptosis and suppresses migration and invasion of highly metastatic lung adenocarcinoma[J]. Carcinogenesis. 2020, 41(5): 699-710.

文献引用

1. Chen L, Zhou X, Kong X, et al. The Prognostic Significance of Anisomycin-Activated Phospho-c-Jun NH2-Terminal Kinase (p-JNK) in Predicting Breast Cancer Patients’ Survival Time. Frontiers in Cell and Developmental Biology. 2021 Mar 9;9:656693. doi: 10.3389/fcell.2021.656693. eCollection 2021. 2. Liu Y J, Chang Y J, Kuo Y T, et al. Targeting β-tubulin/CCT-β complex induces apoptosis and suppresses migration and invasion of highly metastatic lung adenocarcinoma. Carcinogenesis. 2020, 41(5): 699-710 3. Zhang L, Liu W, Wu N, et al.Southern rice black-streaked dwarf virus induces incomplete autophagy for persistence in gut epithelial cells of its vector insect.PLoS pathogens.2023, 19(1): e1011134.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Anisomycin 22862-76-6 Apoptosis Cell Cycle/Checkpoint DNA Damage/DNA Repair MAPK Microbiology/Virology Antibiotic JNK Antibacterial DNA/RNA Synthesis Bacterial Inhibitor 茴香霉素 NSC-76712 Flagecidin NSC 76712 inhibit Wuningmeisu C NSC76712 inhibitor

我们的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

沪公网安备 31010602006700号

陶术

生物

TargetMol^®中国区唯一合作伙伴

点击进入陶术生物官网

联系我们

电话：

400-820-0310

邮箱：

service@tsbiochem.com

QQ：

2881945090（终端用户）

2885109491（经销商）

经销：

终端：

地址：

上海市静安区江场三路238号8楼

Anisomycin

存储

溶解度

溶液配制表

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

文献引用

相关产品

相关化合物库

剂量换算

体内实验配液计算器

技术支持

Keywords