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Ac-DEVD-CHO

Ac-DEVD-CHO

产品编号 TQ0146   CAS 169332-60-9

Ac-DEVD-CHO 是一种特异性 Caspase-3 抑制剂(Ki: 230 pM)。Ac-DEVD-CHO在 SLNT 诱导的细胞凋亡中有抑制作用。

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Ac-DEVD-CHO Chemical Structure
Ac-DEVD-CHO, CAS 169332-60-9
规格 价格/CNY 货期 数量
1 mg ¥ 783 现货
2 mg ¥ 1,150 现货
5 mg ¥ 2,160 现货
10 mg ¥ 3,470 现货
25 mg ¥ 6,520 现货
50 mg ¥ 8,790 现货
100 mg ¥ 11,800 现货
其他形式的 Ac-DEVD-CHO:
产品目录号及名称: Ac-DEVD-CHO (TQ0146)
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Ac-DEVD-CHO is a specific Caspase-3 inhibitor (Ki: 230 pM).Ac-DEVD-CHO has an inhibitory effect in SLNT-induced apoptosis.
靶点活性 Caspase-10:12 nM (Ki), Caspase-1:18 nM(Ki), Caspase-7:1.6 nM(Ki), Caspase-8:0.92 nM(Ki), Caspase 3:0.23 nM(Ki)
体外活性 The addition of Ac-DEVD-CHO significantly prevents SLNT-induced apoptosis (from 32.91% decreases to 15.88% while NC and Ac-DEVD-CHO groups are 6.45%, and 7.77%, respectively) [2].
Ac-DEVD-CHO (10 μmol/L) partially blocks the effect of SIN-induced apoptosis and reduces the number of apoptotic nuclei. These effects of SIN are blocked by the caspase-3 inhibitor Ac-DEVD-CHO. Camptothecin (4 μM), a positive control, increases caspase-3 activity, which is also blocked by Ac-DEVD-CHO.[3]
体内活性 One hundred and two male C57BL/6 mice were subjected to cecal ligation and puncture (CLP, a model of polymicrobial sepsis) or sham operation. The animals were assigned into three equal groups (n=34) according to the random number table: sham group, model group, and caspase-3 inhibitor (CI) group. Thirty minutes before CLP, Ac-DEVD-CHO (4 μg/g) was injected subcutaneously in the CI group. The levels of blood urea nitrogen (BUN) and creatinine (Cr) were determined, and the concentrations of tumor necrosis factor-α (TNF-α), interleukins (IL-6 and IL-10) were measured by enzyme-linked immunosorbent assay (ELISA), the renal cell apoptosis rate was determined by flow cytometry and the expression of caspase-3 mRNA was determined by real-time reverse transcription-polymerase chain reaction (RT-PCR) at 6, 12 and 24 hours after operation. The 4-day and 7-day survival rates of three groups of mice were observed. Results: Compared with the sham group, the concentrations of serum BUN, TNF-α, IL-6, IL-10, and the renal cell apoptosis rates, the caspase-3 mRNA expression were increased significantly at all time points after CLP, the concentrations of serum Cr were increased significantly at 6 hours, with the 4-day and 7-day survival rates were decreased significantly. Compared with the model group, in the CI group, the concentrations of serum BUN were decreased significantly at all time points after operation and those of Cr were decreased significantly at 6 hours, then restored to those of the sham group at 12 hours and 24 hours; the concentrations of serum TNF-α, IL-6 were decreased and those of IL-10 elevated significantly at all time points. (P<0.05); the renal cell apoptosis rate and the expression of caspase-3 mRNA were decreased significantly at all time points. The 4-day survival rate of the CI group was improved (80% vs. 20%), but that of the 7-day did not change (20% vs. 20%).[4]
分子量 502.47
分子式 C20H30N4O11
CAS No. 169332-60-9

存储

keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

H2O: 50.25 mg/mL (100 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.9902 mL 9.9508 mL 19.9017 mL 49.7542 mL
5 mM 0.398 mL 1.9902 mL 3.9803 mL 9.9508 mL
10 mM 0.199 mL 0.9951 mL 1.9902 mL 4.9754 mL
20 mM 0.0995 mL 0.4975 mL 0.9951 mL 2.4877 mL
50 mM 0.0398 mL 0.199 mL 0.398 mL 0.9951 mL
100 mM 0.0199 mL 0.0995 mL 0.199 mL 0.4975 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Garcia-Calvo M, et al.nhibition of human caspases by peptide-based and macromolecular inhibitors. J Biol Chem. 1998 Dec 4;273(49):32608-13. 2. Jinglin Wang, et al. A polysaccharide from Lentinus edodes inhibits human colon cancer cell proliferation and suppresses tumor growth in athymic nude mice. Oncotarget. 2017 Jan 3; 8(1): 610-623. 3. Long-gang He, et al. Sinomenine induces apoptosis in RAW 264.7 cell-derived osteoclasts in vitro via caspase-3 activation. Acta Pharmacol Sin. 2014 Feb; 35(2): 203-210. 4. Liu LX, et al. The effect of caspase-3 inhibitor on the concentrations of serum inflammatory cytokines in sepsis related acute kidney injury induced by peritoneal cavity infection in mice. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Dec;22(12):736-9.
(Rac)-Antineoplaston A10 WHI-P154 Sunitinib Rabeprazole sodium Etidronic acid Fimepinostat Griseofulvin 2’-OMe-Guanosine

相关化合物库

该产品包含在如下化合物库中:
多肽分子库 经典已知活性库 抑制剂库 已知活性化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Ac-DEVD-CHO 169332-60-9 Apoptosis Proteases/Proteasome Caspase AcDEVDCHO Ac DEVD CHO Inhibitor inhibitor inhibit

 

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