Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ACY-775 是一种特异性有效的组蛋白脱乙酰酶 6 抑制剂,IC50值为 7.5 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 393 | 现货 | ||
2 mg | ¥ 583 | 现货 | ||
5 mg | ¥ 972 | 现货 | ||
10 mg | ¥ 1,620 | 现货 | ||
25 mg | ¥ 3,350 | 现货 | ||
50 mg | ¥ 4,880 | 现货 | ||
100 mg | ¥ 6,990 | 现货 | ||
500 mg | ¥ 14,000 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,060 | 现货 |
产品描述 | ACY-775 is an effective and specific inhibitor of HDAC6 (IC50: 7.5 nM). |
靶点活性 | HDAC6:7.5 nM, HDAC1:2123 nM |
体外活性 | Upon treatment with ACY-775, a clear enhancement of the acetylation of α-tubulin is visible, while histone acetylation remains unaltered. Acetylation of α-tubulin is visualized by immunofluorescence and the intensity in the neurites of the neurons is quantified and normalized to the length of the fluorescent signal. In vehicle-treated DRG neurons, acetylated α-tubulin is already present. Upon treatment with ACY-775, the signal intensity of acetylated α-tubulin increases significantly. A significant increase in motility of mitochondria and also the total number of mitochondria within the neurites are observed compared with vehicle-treated DRG neurons. A significantly higher number of retrogradely transport mitochondria is observed in DRG neurons treated with ACY-775 compared with vehicle-treated cells [1]. |
体内活性 | Biodistribution profiles of ACY-775 are examined after acute dosing at 5 or 50?mg/kg over 2?h. At t=30?min after acute 50?mg/kg injection, respective plasma levels of ACY-775 is 1359?ng/mL (4.1?μM). Elimination from the plasma is rapid, with a plasmatic half-life of 12?min and a concentration below 10?ng/mL after 2?h. When ACY-775 (50?mg/kg) is administered repeatedly in wild-type mice at 24?h, 4?h, and 30?min before killing, significant increases in α-tubulin acetylation are observed in all tested brain regions [2]. |
细胞实验 | Undifferentiated RN46A-B14 cells are grown. They are treated with 2.5?μM ACY-738, ACY-775, tubastatin A, 0.6?μM TSA or vehicle (0.1% DMSO) for 4?h. Samples are processed using a histone extraction kit and quantified using protein assay [2]. |
动物实验 | Mice are tested for immobility in the TST. At 30?min or 2?h after i.p. injection of ACY-738 (5, 50?mg/kg), ACY-775 (5, 50?mg/kg), and citalopram (0.5, 2, 20?mg/kg), a combination of the previous, or vehicle, mice are attached to the test rig and time immobile over 6?min is recorded. For open-field activity, mice are injected with ACY-738 or ACY-775 at 5, 10, or 50?mg/kg or vehicle and allowed to explore [2]. |
分子量 | 330.36 |
分子式 | C17H19FN4O2 |
CAS No. | 1375466-18-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 100 mg/mL (302.70 mM), Sonification and heating are recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.027 mL | 15.135 mL | 30.27 mL | 75.675 mL |
5 mM | 0.6054 mL | 3.027 mL | 6.054 mL | 15.135 mL | |
10 mM | 0.3027 mL | 1.5135 mL | 3.027 mL | 7.5675 mL | |
20 mM | 0.1514 mL | 0.7568 mL | 1.5135 mL | 3.7838 mL | |
50 mM | 0.0605 mL | 0.3027 mL | 0.6054 mL | 1.5135 mL | |
100 mM | 0.0303 mL | 0.1514 mL | 0.3027 mL | 0.7568 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ACY-775 1375466-18-4 Chromatin/Epigenetic DNA Damage/DNA Repair HDAC Histone deacetylases inhibit ACY 775 Inhibitor ACY775 inhibitor