Powder: -20°C for 3 years | In solvent: -80°C for 1 year
A 83-01 sodium salt 是一种有效的 TGF-βI 型受体 ALK5,ALK4和 ALK7的抑制剂,抑制 ALK5,ALK4和 ALK7诱导的转录的IC50值分别为 12 nM,45 nM 和 7.5 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 333 | 5日内发货 | ||
5 mg | ¥ 671 | 5日内发货 | ||
10 mg | ¥ 1,090 | 5日内发货 | ||
25 mg | ¥ 1,980 | 5日内发货 |
产品描述 | A 83-01 sodium salt is a potent inhibitor of TGF-β type I receptor ALK5 kinase, ALK4 and ALK7, with IC 50 s of 12 nM, 45 nM and 7.5 nM against the ALK5, ALK4 and ALK7 induced transcription, respectively [1]. |
靶点活性 | ALK7 (cell-based assay):7.5 nM , ALK5 (cell-based assay):12 nM , ALK4 (cell-based assay):45 nM |
体外活性 | A 83-01 sodium salt is a potent inhibitor of TGF-β type I receptor ALK5 kinase, type I activin/nodal receptor ALK4 and type I nodal receptor ALK7, reduces the level of ALK-5-induced transcription with an IC 50 of 12 nM in Mv1Lu cells, also blocks the ALK4-TD and ALK7-TD induced transcription with IC 50 s of 45 nM and 7.5 nM in R4-2 cells, and weakly suppresses that induced by constitutively active ALK-6, ALK-2, ALK-3, and ALK-1. A 83-01 (0.03-10 μM) potently prevents the growth-inhibitory effects of TGF-β, and completely inhibits the effect at 3 μM. A 83-01 (1-10 μM) inhibits TGF-β-induced Smad activation in HaCaT cells [1]. A 83-01 (1 μM) decreases cell motility, adhesion and invasion increased by TGF-β1 in HM-1 cells while not change cell proliferation [2]. |
体内活性 | A 83-01 (50, 150 and 500 μg/mouse, i.p.) markedly improves survival of the mice without body weight or neurobehavioral appearances [2]. A 83-01 (0.5 mg/kg, i.p.) shows a significantly strong antitumor effect in mice bearing M109 cells [3]. |
分子量 | 444.51 |
分子式 | C25H19N5NaS+ |
CAS No. | 2828431-89-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 100 mg/mL (224.97 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.2497 mL | 11.2483 mL | 22.4967 mL | 56.2417 mL |
5 mM | 0.4499 mL | 2.2497 mL | 4.4993 mL | 11.2483 mL | |
10 mM | 0.225 mL | 1.1248 mL | 2.2497 mL | 5.6242 mL | |
20 mM | 0.1125 mL | 0.5624 mL | 1.1248 mL | 2.8121 mL | |
50 mM | 0.045 mL | 0.225 mL | 0.4499 mL | 1.1248 mL | |
100 mM | 0.0225 mL | 0.1125 mL | 0.225 mL | 0.5624 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
A 83-01 sodium salt 2828431-89-4 Angiogenesis Tyrosine Kinase/Adaptors ALK A 83 01 sodium salt A 8301 sodium salt A 83-01 sodium Inhibitor inhibitor inhibit