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6-Aminonicotinamide

6-Aminonicotinamide

产品编号 T7545   CAS 329-89-5

6-Aminonicotinamide 是一种竞争性 NADP+依赖性酶,是一种烟酰胺抗代谢药,葡萄糖 6-磷酸脱氢酶抑制剂 (Ki=0.46 μM)。 它干扰糖酵解,导致 ATP 耗竭,并与 DNA 交联化学疗法药物,如Cisplatin,协同杀死癌细胞。

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6-Aminonicotinamide Chemical Structure
6-Aminonicotinamide, CAS 329-89-5
规格 价格/CNY 货期 数量
50 mg ¥ 289 现货
100 mg ¥ 413 现货
200 mg ¥ 588 现货
1 mL * 10 mM (in DMSO) ¥ 455 现货
千万补贴 助力科研
BCA蛋白浓度测定试剂盒限时半价
重组蛋白限时优惠
产品目录号及名称: 6-Aminonicotinamide (T7545)
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纯度: 98%
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生物活性
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存储 & 溶解度
参考文献
产品描述 6-Aminonicotinamide (6-AN) is a well-established inhibitor of the NADP+-dependent enzyme.
体外活性 The EC50 of 6-AN on the inhibition of surface antigen (HBsAg) is approximately 23.89 μM in HepAD38 cells with a sensitive index (SI) > 20[1]
体内活性 6-AN decreases HBsAg production but not blocks its secretion[1].
细胞实验 The cytotoxic effects of 6-AN on various cells were assessed by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay .?Cells were seeded into 96-well?plates and incubated with different concentrations (multiple proportion dilution from 500?μM to 0.98?μM) of 6-AN for 72 h. Next, 40?ug/ml MTT were added into cell culture media and incubated for 4 h under the condition of protection from light.?After that, dimethyl sulfoxide (DMSO) (Solarbio) was added and incubated overnight in the dark to lyses the cells and solubilize formazan dissolving in media.?Fifty-percent cytotoxicity concentrations (CC50) and Fifty-percent effective concentrations (EC50) were determined with a microplate reader and calculated via non-linear regression using GraphPad Prism 5.0[1].
动物实验 HBV-transgenic mice (HBV-Tg C57 BL/6),Mice were selected as age (6 to 8 weeks), weight (21±1 g), and possess of basically the same serum HBsAg and HBV DNA levels.?Next, the mice were assigned to four groups of 10 individuals per group at random: negative control (0.9% saline), positive control (0.02 mg/kg Entecavir (ETV)), low concentration test group (6-AN 2.5 mg/kg), high concentration test group (6-AN 5 mg/kg).?Animals received 0.9% saline or 6-AN via intraperitoneal injection and animals received ETV via oral gavage every two days.?The serum samples were collected via orbital blood every four days after injection.?All blood was collected for analysis of serum HBsAg and HBV markers.?6 mice from each group were sacrificed by cervical dislocation at day 20 and the liver samples were used for intrahepatic HBV DNA and RNA determination.?The remaining animals were monitored without treatment up to day 32[1].
分子量 137.14
分子式 C6H7N3O
CAS No. 329-89-5

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 27.5 mg/mL (200.52 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 7.2918 mL 36.4591 mL 72.9182 mL 182.2955 mL
5 mM 1.4584 mL 7.2918 mL 14.5836 mL 36.4591 mL
10 mM 0.7292 mL 3.6459 mL 7.2918 mL 18.2295 mL
20 mM 0.3646 mL 1.823 mL 3.6459 mL 9.1148 mL
50 mM 0.1458 mL 0.7292 mL 1.4584 mL 3.6459 mL
100 mM 0.0729 mL 0.3646 mL 0.7292 mL 1.823 mL

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TargetMol Library Books参考文献

1. Ren F, Yang X, Hu ZW,et al.Niacin analogue, 6-Aminonicotinamide, a novel inhibitor of hepatitis B virus replication and HBsAg production[J].EBioMedicine. 2019 Nov;49:232-246. 2. Shawgi, Hago, Almugadam, et al. Influence of 6-aminonicotinamide (6AN) on Leishmania promastigotes evaluated by metabolomics: Beyond the pentose phosphate pathway.[J]. Chemico-biological interactions, 2018.

TargetMol Library Books文献引用

1. Li M, Yang J, Ye C, et al. Integrated Metabolomics and Transcriptomics Analyses Reveal Metabolic Landscape in Neuronal Cells during JEV Infection. Virologica Sinica. 2021: 1-12.

相关化合物库

该产品包含在如下化合物库中:
抗癌活性化合物库 抑制剂库 人代谢物化合物库 已知活性化合物库 NO PAINS 化合物库 抗癌化合物库 经典已知活性库

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母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

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Keywords

6-Aminonicotinamide 329-89-5 Others reactions DNA-crosslinking Inhibitor 6Aminonicotinamide ATP redox 6 Aminonicotinamide antimetabolite nicotinamide inhibit depletion inhibitor

 

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