Powder: -20°C for 3 years | In solvent: -80°C for 1 year
6-Aminonicotinamide 是一种竞争性 NADP+依赖性酶,是一种烟酰胺抗代谢药,葡萄糖 6-磷酸脱氢酶抑制剂 (Ki=0.46 μM)。 它干扰糖酵解,导致 ATP 耗竭,并与 DNA 交联化学疗法药物,如Cisplatin,协同杀死癌细胞。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 289 | 现货 | ||
100 mg | ¥ 413 | 现货 | ||
200 mg | ¥ 588 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 455 | 现货 |
产品描述 | 6-Aminonicotinamide (6-AN) is a well-established inhibitor of the NADP+-dependent enzyme. |
体外活性 | The EC50 of 6-AN on the inhibition of surface antigen (HBsAg) is approximately 23.89 μM in HepAD38 cells with a sensitive index (SI) > 20[1] |
体内活性 | 6-AN decreases HBsAg production but not blocks its secretion[1]. |
细胞实验 | The cytotoxic effects of 6-AN on various cells were assessed by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay .?Cells were seeded into 96-well?plates and incubated with different concentrations (multiple proportion dilution from 500?μM to 0.98?μM) of 6-AN for 72 h. Next, 40?ug/ml MTT were added into cell culture media and incubated for 4 h under the condition of protection from light.?After that, dimethyl sulfoxide (DMSO) (Solarbio) was added and incubated overnight in the dark to lyses the cells and solubilize formazan dissolving in media.?Fifty-percent cytotoxicity concentrations (CC50) and Fifty-percent effective concentrations (EC50) were determined with a microplate reader and calculated via non-linear regression using GraphPad Prism 5.0[1]. |
动物实验 | HBV-transgenic mice (HBV-Tg C57 BL/6),Mice were selected as age (6 to 8 weeks), weight (21±1 g), and possess of basically the same serum HBsAg and HBV DNA levels.?Next, the mice were assigned to four groups of 10 individuals per group at random: negative control (0.9% saline), positive control (0.02 mg/kg Entecavir (ETV)), low concentration test group (6-AN 2.5 mg/kg), high concentration test group (6-AN 5 mg/kg).?Animals received 0.9% saline or 6-AN via intraperitoneal injection and animals received ETV via oral gavage every two days.?The serum samples were collected via orbital blood every four days after injection.?All blood was collected for analysis of serum HBsAg and HBV markers.?6 mice from each group were sacrificed by cervical dislocation at day 20 and the liver samples were used for intrahepatic HBV DNA and RNA determination.?The remaining animals were monitored without treatment up to day 32[1]. |
分子量 | 137.14 |
分子式 | C6H7N3O |
CAS No. | 329-89-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 27.5 mg/mL (200.52 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 7.2918 mL | 36.4591 mL | 72.9182 mL | 182.2955 mL |
5 mM | 1.4584 mL | 7.2918 mL | 14.5836 mL | 36.4591 mL | |
10 mM | 0.7292 mL | 3.6459 mL | 7.2918 mL | 18.2295 mL | |
20 mM | 0.3646 mL | 1.823 mL | 3.6459 mL | 9.1148 mL | |
50 mM | 0.1458 mL | 0.7292 mL | 1.4584 mL | 3.6459 mL | |
100 mM | 0.0729 mL | 0.3646 mL | 0.7292 mL | 1.823 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
6-Aminonicotinamide 329-89-5 Others reactions DNA-crosslinking Inhibitor 6Aminonicotinamide ATP redox 6 Aminonicotinamide antimetabolite nicotinamide inhibit depletion inhibitor