Powder: -20°C for 3 years | In solvent: -80°C for 1 year
5a-Pregnane-3,20-dione 是内源性的孕酮代谢物。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 mg | ¥ 163 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 150 | 现货 |
产品描述 | 5a-Pregnane-3,20-dione is a biologically active 5-alpha-reduced metabolite of plasma progesterone |
体外活性 | the endogenous progesterone metabolite,5a-Pregnane-3,20-dione, may be involved in promoting breast neoplasia and metastasis by affecting adhesion and cytoskeletal molecules. |
细胞实验 | MCF-7 cells were grown without or with 5a-Pregnane-3,20-dione (10(-9)-10(-5) M), and the effects on cell and nuclear morphology, adhesion plaques, vinculin and actin expression, actin polymerization, and microfilament distribution were examined by immunohistochemistry, morphometry (using confocal microscopy and digital computer imaging analysis), and Western blotting.?Treatment of cells with 10(-9)-10(-6) M 5a-Pregnane-3,20-dione resulted in dose-dependent decreases in cell area, cell-to-cell contacts, and attachment to the substratum, and increases in variation in nuclear area.?These changes in the 5alphaP-treated cells were accompanied by decreases in vinculin-containing adhesion plaques, vinculin expression, polymerized actin stress fibers, and decreases in insoluble and increases in soluble actin fractions. |
分子量 | 316.48 |
分子式 | C21H32O2 |
CAS No. | 566-65-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 5 mg/mL (15.80 mM), sonification/heating is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.1598 mL | 15.7988 mL | 31.5976 mL | 78.9939 mL |
5 mM | 0.632 mL | 3.1598 mL | 6.3195 mL | 15.7988 mL | |
10 mM | 0.316 mL | 1.5799 mL | 3.1598 mL | 7.8994 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
5a-Pregnane-3,20-dione 566-65-4 Metabolism Endogenous Metabolite Inhibitor inhibit 5aPregnane3,20dione 5a Pregnane 3,20 dione inhibitor