Powder: -20°C for 3 years | In solvent: -80°C for 1 year
360A 是一种稳定的G-四链体配体,还抑制 TRAP-G4 试验中端粒酶的端粒酶活性,IC50值为 300 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 935 | 现货 | ||
2 mg | ¥ 1,490 | 现货 | ||
5 mg | ¥ 2,130 | 现货 | ||
10 mg | ¥ 3,260 | 现货 | ||
25 mg | ¥ 5,330 | 现货 | ||
50 mg | ¥ 7,520 | 现货 | ||
100 mg | ¥ 9,870 | 现货 |
产品描述 | 360A is a stabilizing G-Quadruplex ligand, and also inhibits telomerase activity for telomerase in TRAP-G4 assay(IC50 : 300 nM). |
靶点活性 | Telomerase:300 nM |
体外活性 | A549 lung carcinoma cell lines treatment with 5 μM of 360A led to an inhibition of cell proliferation within 11 days of treatmen. Treatment with the newly synthesized dimer (360A)2A led to a comparable antiproliferative effect[1] |
细胞实验 | Supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin. A549 cells were maintained in humidified incubators at 37 °C in an atmosphere containing 5% CO2 and ambient oxygen of 20%. Cells were grown in 25 cm^2 flasks (150,000 cells/flask) and treated with 5 μM of 360A or (360A)2A every 3 or 4 days (i.e., at each reseeding) until cultures were terminated. Control cells were either non-treated or treated with 0.1% DMSO. At each reseeding the remaining cells were pelleted and snap frozen for further DNA extraction.Statistical analysis was performed using GraphPad Prism 5. A Chi-square test was used to compare TDEs frequencies[1]. |
别名 | 3,3'-[2,6-吡啶二基二(羰基亚氨基)]二[1-甲基喹啉鎓] |
分子量 | 449.5 |
分子式 | C27H23N5O2 |
CAS No. | 794458-56-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: Slightly soluble
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
360A 794458-56-3 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis Telomerase 3,3'-[2,6-吡啶二基二(羰基亚氨基)]二[1-甲基喹啉鎓] inhibit Inhibitor G-quadruplex inhibitor