Powder: -20°C for 3 years | In solvent: -80°C for 1 year
20S Proteasome activator 1 是一种 20S 蛋白酶激活剂,对胰蛋白酶样位点、胰凝乳蛋白酶样位点和半胱天冬酶位点的 IC50 分别为 0.3 μM、0.7 μM 和 1.8 μM。20S Proteasome activator 1 通过在细胞系统中翻译来防止致病性 α-synuclein A53T 突变体积累从而减少疾病发生的概率。20S Proteasome activator 1 可用于研究神经退行性疾病。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 281 | 现货 | ||
5 mg | ¥ 663 | 现货 | ||
10 mg | ¥ 985 | 现货 | ||
25 mg | ¥ 1,960 | 现货 | ||
50 mg | ¥ 3,320 | 现货 | ||
100 mg | ¥ 4,850 | 现货 | ||
500 mg | ¥ 9,870 | 现货 |
产品描述 | 20S Proteasome activator 1 is a 20S proteasome activator with IC50 of 0.3 μM for trypsin-like sites, 0.7 μM for chymotrypsin-like sites and 1.8 μM for caspase sites. 20S Proteasome activator 1 prevents the accumulation of pathogenic alpha-synuclein A53T mutants through translation in the cellular system, thereby reducing the probability of disease occurrence. The 20S Proteasome activator 1 can be used to study neurodegenerative diseases. |
靶点活性 | Caspase-like site:1.8 μM, Trypsin like site:0.3 μM, Chymotrypsin-like site:0.7 μM |
体外活性 |
At concentrations of 2.5-15 μM and a treatment duration of 45 minutes, 20S Proteasome activator 1 (compound 19) degrades 73% of α-synuclein at 10 μM and over 80% at 15 μM.[1] At concentrations of 5-15 μM and a 24-hour treatment period, 20S Proteasome activator 1 reduces A53T mutant α-synuclein by 25%. It also exhibits a dose-dependent reduction in the augmentation of α-synuclein in Hek-293T cells transiently transfected with an A53T mutant α-synuclein plasmid, achieving a 67% decrease at 15 μM.[1] |
分子量 | 492.97 |
分子式 | C27H19ClF2N2OS |
CAS No. | 2761578-18-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 49.30 mg/mL (100 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.0285 mL | 10.1426 mL | 20.2852 mL | 50.713 mL |
5 mM | 0.4057 mL | 2.0285 mL | 4.057 mL | 10.1426 mL | |
10 mM | 0.2029 mL | 1.0143 mL | 2.0285 mL | 5.0713 mL | |
20 mM | 0.1014 mL | 0.5071 mL | 1.0143 mL | 2.5357 mL | |
50 mM | 0.0406 mL | 0.2029 mL | 0.4057 mL | 1.0143 mL | |
100 mM | 0.0203 mL | 0.1014 mL | 0.2029 mL | 0.5071 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
20S Proteasome activator 1 2761578-18-9 Proteases/Proteasome Ubiquitination Proteasome Inhibitor inhibitor inhibit