Powder: -20°C for 3 years | In solvent: -80°C for 1 year
10,12-Tricosadiynoic acid 是一种口服有效的酰基辅酶 A 氧化酶-1 (ACOX1) 抑制剂,具有高特异性,选择性,高亲和力的特点。10,12-Tricosadiynoic acid 可改善线粒体脂质和 ROS 代谢,在高脂饮食或肥胖引起的代谢性疾病中有研究的价值。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 255 | 现货 | ||
10 mg | ¥ 417 | 现货 | ||
25 mg | ¥ 671 | 现货 | ||
50 mg | ¥ 925 | 现货 | ||
100 mg | ¥ 1,178 | 现货 | ||
500 mg | ¥ 2,960 | 现货 |
产品描述 | 10,12-Tricosadiynoic acid is a highly selective and orally active inhibitor of acyl-CoA oxidase-1 (ACOX1). It can treat the high-fat diet- or obesity-induced metabolic diseases by improving mitochondrial lipid and ROS metabolism. |
体外活性 | 10,12-Tricosadiynoic acid-CoA rapidly inhibits ACOX1 activity in a time- and concentration-dependent manner. The activity of ACOX1 decreases by nearly 95% after 5 min of incubation with 10 eq of 10,12-Tricosadiynoic acid-CoA. ACOX1 activity is inhibited only if free 10,12-Tricosadiynoic Acid is activated as the CoA thioester, the substrate form. The inhibition of ACOX1 by 10,12-Tricosadiynoic acid-CoA is irreversible. And the kinetics parameters KI and kinact are calculated to be 680 nm and 3.18/min, respectively. |
体内活性 | 10,12-Tricosadiynoic acid (100 μg/kg; oral gavage; daily; for 8 weeks; male Wistar rats) treatment increases hepatic mitochondrial fatty acid oxidation (FAO) via activation of the SIRT1-AMPK pathway and proliferator activator receptor α and reduces hydrogen peroxide accumulation in high fat diet-fed rats, which significantly decreases hepatic lipid and ROS contents, reduces body weight gain, and decreases serum triglyceride and insulin levels. |
动物实验 | Animal Model: Male Wistar rats (210-230 g) fed with high-fat diet. Dosage: 100 μg/kg. Administration: Oral gavage; daily; for 8 weeks |
别名 | TDA, TCDA |
分子量 | 346.55 |
分子式 | C23H38O2 |
CAS No. | 66990-30-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 2.75 mg/mL (7.94 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.8856 mL | 14.4279 mL | 28.8559 mL | 72.1397 mL |
5 mM | 0.5771 mL | 2.8856 mL | 5.7712 mL | 14.4279 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
10,12-Tricosadiynoic acid 66990-30-5 Metabolism Acyltransferase ROS mono- acylglycerol acyltransferase 10,12Tricosadiynoic acid fatty Diacylglycerol acyltransferase TDA SIRT1-AMPK Diglyceride acyltransferase metabolic mitochondrial oxidation insulin 10,12 Tricosadiynoic acid inhibit triglyceride Inhibitor acyl-CoA:cholesterol acyltransferase TCDA acid inhibitor