Powder: -20°C for 3 years | In solvent: -80°C for 1 year
1-Azakenpaullone (azakenpaullone) 是一种 ATP 竞争性的、具有高度选择性的糖原合成酶激酶3 β (GSK-3β)的抑制剂,其 IC50=18 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 497 | 现货 | ||
2 mg | ¥ 747 | 现货 | ||
5 mg | ¥ 1,660 | 现货 | ||
10 mg | ¥ 2,660 | 现货 | ||
25 mg | ¥ 4,890 | 现货 | ||
50 mg | ¥ 6,930 | 现货 | ||
100 mg | ¥ 9,530 | 现货 | ||
500 mg | ¥ 18,900 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,780 | 现货 |
产品描述 | 1-Azakenpaullone (azakenpaullone) is a potent and selective GSK-3β inhibitor with IC50 of 18 nM, >100-fold selectivity over CDK1/cyclin B and CDK5/p25. |
靶点活性 | GSK-3β:18 nM |
体外活性 | 1-Azakenpaullone inhibits the CDK1/cyclin B, CDK5/p25, and GSK-3β effectively, with IC50 of 0.018 μM, 4.2 μM, and 2.0 μM, respectively. [1] In human islets, 1-Azakenpaullone (5 mM) in combination with glucose (8 mM) stimulates the β-cell proliferation. [2] 1-Azakenpaullone effectively stimulates INS-1E cells replication and protects INS-1E cells against glucolipotoxicity-induced cell death. [3][4] |
体内活性 | Pretreatment with 1-Azakenpaullone (10 or 100 pmol, i.c.v.) attenuates the ketamine-induced locomotor hyperactivity, disruption of PPI and cognitive de?cits, and improves the ketamine-induced motor incoordination in rotarod test. [5] |
激酶实验 | Kinase preparations and assays: GSK-3β is assayed, following a 1/100 dilution in 1 mg BSA per mL 10 mM dithiothreitol, with 5 μL 40 μM GS-1 peptide as a substrate, in buffer A, in the presence of 15 μM [γ-32P]ATP (3000 Ci·mmol-1; 1 mCi·mL-1 ) in a final volume of 30 μL. After 30 min incubation at 30℃, 25 μL aliquots of supernatant are spotted onto 2.5×3 cm pieces of Whatman P81 phosphocellulose paper, and 20 s later, the filters are washed five times in a solution of 10 mL phosphoric acid per L of water. The wet filters are counted in the presence of 1 mL ACS scintillation fluid. The kinase activity of CDK1/cyclin B is assayed in buffer C, with 1 mg/mL histone H1, in the presence of 15 μM [γ-32P]ATP (3000 Ci·mmol-1; 1 mCi·mL-1 ) in a final volume of 30 μL. After 10 min incubation at 30℃, 25 μL aliquots of supernatant are spotted onto P81 phosphocellulose papers and treated as described above. The activity of CDK5/p25 is assayed in buffer C as described for CDK1/cyclin B. (Buffer A: 10 mM MgCl2 , 1 mM EGTA, 1 mM dithiothreitol, 25 mM Tris/HCl pH 7.5, 50 μg heparin/mL. Buffer C: homogenization buffer but 5 mM EGTA, no NaF and no protease inhibitors.) |
细胞实验 | Cell replication is determined by BrdUrd incorporation after treatment with 1-Azakenpaullone for 24 h. The relative cell number is determined after treatment with 1-Azakenpaullone for 4 days using the CyQuant cell proliferation assay. Results are presented as fold change relative to control. (Only for Reference) |
别名 | 1-氮杂坎帕罗酮, azakenpaullone |
分子量 | 328.16 |
分子式 | C15H10BrN3O |
CAS No. | 676596-65-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 61 mg/mL (185.9 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.0473 mL | 15.2365 mL | 30.4729 mL | 76.1824 mL |
5 mM | 0.6095 mL | 3.0473 mL | 6.0946 mL | 15.2365 mL | |
10 mM | 0.3047 mL | 1.5236 mL | 3.0473 mL | 7.6182 mL | |
20 mM | 0.1524 mL | 0.7618 mL | 1.5236 mL | 3.8091 mL | |
50 mM | 0.0609 mL | 0.3047 mL | 0.6095 mL | 1.5236 mL | |
100 mM | 0.0305 mL | 0.1524 mL | 0.3047 mL | 0.7618 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
1-Azakenpaullone 676596-65-9 PI3K/Akt/mTOR signaling Stem Cells GSK-3 Glycogen synthase kinase-3 1 Azakenpaullone 1-氮杂坎帕罗酮 1Azakenpaullone Glycogen synthase kinase 3 azakenpaullone Inhibitor inhibit 1-Akp inhibitor