Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DOPE (DOPE) 是一种阳离子脂质体的中性辅助脂质 (helper lipid),能够与阳离子磷脂结合,增强裸 siRNA 的转染效率。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 197 | 现货 | ||
100 mg | ¥ 987 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 587 | 现货 |
产品描述 | DOPE (DOPE) is a neutral helper lipid for cationic liposomes.It is used in combination with cationic phospholipids to increase efficiency during DNA transfection studies as a non-viral method of gene delivery. |
靶点活性 | Helper lipid for liposome: |
体外活性 | Each siRNA is encapsulated in LNP (cationic lipid-A: PEG2000-DMPE: DOPE = 47.8: 5.18: 47.1 molar percent). With the neutral lipid for the LNP is DOPE, LNP exerts knock-down (KD) effect on macrophages and DCs and Liposome-containing DOPE shows fusogenicity. LNP/siRNA significantly suppresses CD45 protein expression at an inhibition rate of 30% on macrophages and DCs but not on monocytes, neutrophils, plasmacytoid DCs, or B cells. |
别名 | 1,2-二油酰-SN-甘油-3-磷酰乙醇胺 |
分子量 | 744.03 |
分子式 | C41H78NO8P |
CAS No. | 4004-05-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 31.25 mg/mL (42.00 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.344 mL | 6.7202 mL | 13.4403 mL | 33.6008 mL |
5 mM | 0.2688 mL | 1.344 mL | 2.6881 mL | 6.7202 mL | |
10 mM | 0.1344 mL | 0.672 mL | 1.344 mL | 3.3601 mL | |
20 mM | 0.0672 mL | 0.336 mL | 0.672 mL | 1.68 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
DOPE 4004-05-1 Others DNA emulsifier 1,2-二油酰-SN-甘油-3-磷酰乙醇胺 1,2 Dioleoyl sn glycero 3 phosphoethanolamine siRNA dioleoylphosphatidylethanolamine phospholipid inhibit knock-down liposome Inhibitor cationic 1,2Dioleoylsnglycero3phosphoethanolamine transfection inhibitor