Powder: -20°C for 3 years | In solvent: -80°C for 1 year
(-)-Butin (Butin) 是 Butin 的 S 对映体。其中 Butin 是一种分离自黄檀的心材中的生物活性黄酮类化合物,具有显著的抗血小板、抗氧化、抗炎作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 756 | 现货 | ||
5 mg | ¥ 1,590 | 现货 | ||
10 mg | ¥ 2,370 | 现货 | ||
25 mg | ¥ 3,970 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,520 | 现货 |
产品描述 | (-)-Butin (Butin) has antioxidant activity, can protect cells against H2O2-induced apoptosis, oxidative DNA damage and oxidative mitochondrial dysfunction; it attenuates oxidative stress by activating Nrf2-mediated Mn SOD induction via the PI3K/Akt signaling pathway. |
体外活性 | The antioxidant function of manganese superoxide dismutase (Mn SOD) is important in preventing oxidative stress. While exposure to H2O2 reduced the expression of Mn SOD in Chinese hamster lung fibroblast (V79-4), the addition of Butin restored Mn SOD expression at both the mRNA and protein levels, resulting in increased Mn SOD activity. The transcription factor NF-E2-related factor 2 (Nrf2) regulates Mn SOD gene expression by binding to the antioxidant responsive element (ARE). Butin enhanced the nuclear translocation and ARE-binding activity of Nrf2, which was decreased by H2O2. The siRNA-mediated knockdown of Nrf2 attenuated Butin-induced Mn SOD expression and activity. Further, phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB, Akt) contributed to the ARE-driven Mn SOD expression. Butin activated PI3K/Akt and exposure to either LY294002 (a PI3K inhibitor), Akt inhibitor IV (an Akt-specific inhibitor), or Akt siRNA suppressed the Butin-induced activation of Nrf2, resulting in decreased Mn SOD expression and activity. Finally, the cytoprotective effect of Butin against H2O2-induced cell damage was suppressed by the siRNA-mediated knockdown of Mn SOD[1] |
别名 | Butin, 漆黄素 ( 紫铆素) |
分子量 | 272.25 |
分子式 | C15H12O5 |
CAS No. | 492-14-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (183.65 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.6731 mL | 18.3655 mL | 36.7309 mL | 91.8274 mL |
5 mM | 0.7346 mL | 3.6731 mL | 7.3462 mL | 18.3655 mL | |
10 mM | 0.3673 mL | 1.8365 mL | 3.6731 mL | 9.1827 mL | |
20 mM | 0.1837 mL | 0.9183 mL | 1.8365 mL | 4.5914 mL | |
50 mM | 0.0735 mL | 0.3673 mL | 0.7346 mL | 1.8365 mL | |
100 mM | 0.0367 mL | 0.1837 mL | 0.3673 mL | 0.9183 mL |
中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
刺槐花 | Robinia pseudoacacia L. | 平 | 甘 | 肝 |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
(-)-Butin 492-14-8 Cytoskeletal Signaling Immunology/Inflammation PI3K/Akt/mTOR signaling Akt PI3K Nrf2 inhibit Inhibitor Butin ()Butin 漆黄素 ( 紫铆素) ( ) Butin inhibitor