keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
(-)-Alkannin (Shikonin) 是从紫朱草中发现的一种天然产物,具有抗癌活性,可抑制细胞周期,诱导细胞凋亡,用作食品着色剂,在 Rho 激酶途径中可改善肝脏炎症。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 mg | ¥ 360 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 260 | 现货 |
产品描述 | (-)-Alkannin (Shikonin) is a natural red naphthoquinone pigment, has antimicrobial, anti-tumor, and anti-inflammatory effects; a purified shikonin preparation is widely used for the production of medicinals, cosmetics, and some food products; shikonin also enters into the antiinflammatory ointment and cream compositions used for the treatment of burns. It can suppress the cell viability, adhesion, invasion and migratory ability of MGC-803 cells through TLR2- or NF-κB-mediated pathway. |
体外活性 | 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed for cell viability and adhesion ability of MGC-803 cells. Cell scratch repair experiments were conducted for the determination of migration ability while transwell assay for cell invasion ability. Western blot analysis and real-time polymerase chain reaction assay were used for the detection of protein and mRNA expressions. Fifty per cent inhibitory concentration of Shikonin on MGC-803 cells was 1.854?μm. Shikonin (1?μm) inhibited significantly the adhesion, invasion and migratory ability of MGC-803 cells. Interestingly, Shikonin in the presence or absence of anti-Toll-like receptor 2 (TLR2) antibody (2?μg) and nuclear factor-kappa B (NF-κB) inhibitor MG-132 (10?μm) could decrease these ability of MGC-803 cells markedly, as well as the expression levels of matrix metalloproteinases (MMP)-2, MMP-7, TLR2 and p65 NF-κB. In addition, the co-incubation of Shikonin and anti-TLR2/MG-132 has a significant stronger activity than anti-TLR2 or MG-132 alone. |
激酶实验 | Histamine release from basophils stimulated with anti-IgE antibody was analyzed fluorimetrically. Syk and Lyn kinase activities were tested in Vitro with recombinant proteins and analyzed by off-chip mobility shift assay. Shikonin dose-dependently inhibited the histamine release from basophils induced by anti-IgE antibody (IC50 = 2.6 +/- 1.0 microM; mean +/- SEM). A search for the target(s) of Shikonin in the signal cascade of IgE-mediated activation showed that it strongly inhibits Syk (IC50 = 7.8 microM, in the recombinant kinase assay), which plays a pivotal role in the degranulation response. A less significant inhibition was found for Lyn, which phosphorylates FcepsilonRI-betagamma subunits and also Syk. |
别名 | Alkannin, 左旋紫草素, 紫草素, Shikonin |
分子量 | 288.3 |
分子式 | C16H16O5 |
CAS No. | 517-88-4 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (173.43 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.4686 mL | 17.343 mL | 34.6861 mL | 86.7152 mL |
5 mM | 0.6937 mL | 3.4686 mL | 6.9372 mL | 17.343 mL | |
10 mM | 0.3469 mL | 1.7343 mL | 3.4686 mL | 8.6715 mL | |
20 mM | 0.1734 mL | 0.8672 mL | 1.7343 mL | 4.3358 mL | |
50 mM | 0.0694 mL | 0.3469 mL | 0.6937 mL | 1.7343 mL | |
100 mM | 0.0347 mL | 0.1734 mL | 0.3469 mL | 0.8672 mL |
中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
软紫草 | Arnebiaeuchroma(Royle) Johnst. | 寒 | 苦 | 心包, 肝 |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
(-)-Alkannin 517-88-4 Apoptosis PI3K/Akt/mTOR signaling PI3K Inhibitor ()Alkannin Alkannin 左旋紫草素 紫草素 inhibit Shikonin ( ) Alkannin inhibitor