Powder: -20°C for 3 years | In solvent: -80°C for 1 year
(-)-Blebbistatin ((S)-(-)-Blebbistatin) 是 blebbistatin 的 S 对映异构体。它是一种有效的选择性肌球蛋白 II 抑制剂,IC50 范围为 0.5 至 5 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 226 | 现货 | ||
2 mg | ¥ 313 | 现货 | ||
5 mg | ¥ 588 | 现货 | ||
10 mg | ¥ 822 | 现货 | ||
25 mg | ¥ 1,630 | 现货 | ||
50 mg | ¥ 2,130 | 现货 | ||
100 mg | ¥ 2,970 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 568 | 现货 |
产品描述 | (-)-Blebbistatin ((S)-(-)-Blebbistatin) is an S enantiomer of blebbistatin. It is a potent and selective myosin II inhibitor with IC50 ranging from 0.5 to 5 μM. |
靶点活性 | Non-muscle myosin II ATPases:0.5 μM-5 μM |
体内活性 | Blebbistatin completely relaxes both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction in a dose-dependent manner. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz[4]. |
激酶实验 | Inhibition of Cox Activity: Inhibition of Cox-1 activity is measured by monitoring oxygen consumption during the conversion of arachidonic acid to PGs using a Clark-type O2-electrode. The reaction mixture contains ~0.2 units Cox-1 in 100 μL of microsome fraction derived from ram seminal vesicles as a crude source of Cox-1 (specific activity 0.2–1 units/mg protein) or 0.23 units of recombinant human Cox-2 (specific activity 43 units/mg protein). For calculation, the rate of O2 consumption is compared with a DMSO control (100% activity). Piroxicam, a nonsteroidal anti-inflammatory drug, is used as positive inhibitory substance for Cox-1 activity with an IC50 of 0.35 ± 0.05 μM (n = 2). Alternatively, nimesulide, a Cox-2 specific inhibitor, inhibits Cox-2 activity by 52 ± 5.7% (n = 2) at a concentration of 50 μM. |
细胞实验 | Freshly isolated HSCs are replated on 96-well plate. At day 3, medium is replaced by serum-free medium and cells are starved overnight, treated with or without blebbistatin (25 μM) for 2 h followed by stimulation with platelet-derived growth factor-BB (20 ng/mL). After an overnight incubation, the WST-1 cell proliferation assay are performed[3]. |
别名 | (S)-(-)-Blebbistatin |
化合物与蛋白结合的复合物 |
Crystal Structures of (S)-(-)-Blebbistatin Analogs bound to Dictyostelium discoideum myosin II |
分子量 | 292.33 |
分子式 | C18H16N2O2 |
CAS No. | 856925-71-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 29.2 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.4208 mL | 17.104 mL | 34.2079 mL | 85.5198 mL |
5 mM | 0.6842 mL | 3.4208 mL | 6.8416 mL | 17.104 mL | |
10 mM | 0.3421 mL | 1.7104 mL | 3.4208 mL | 8.552 mL | |
20 mM | 0.171 mL | 0.8552 mL | 1.7104 mL | 4.276 mL | |
50 mM | 0.0684 mL | 0.3421 mL | 0.6842 mL | 1.7104 mL | |
100 mM | 0.0342 mL | 0.171 mL | 0.3421 mL | 0.8552 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
(-)-Blebbistatin 856925-71-8 Cytoskeletal Signaling Membrane transporter/Ion channel ATPase Myosin inhibit (S)-(-)-Blebbistatin Blebbistatin Inhibitor ( ) Blebbistatin ()Blebbistatin inhibitor