Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF. PDGF R alpha/PDGFRA Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 57.32 kDa and the accession number is Q0PW86.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | ||
500 μg | ¥ 12,400 | 5日内发货 | ||
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 | Immobilized Cynomolgus PDGF R alpha, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. |
产品描述 | Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF. PDGF R alpha/PDGFRA Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 57.32 kDa and the accession number is Q0PW86. |
种属 | Cynomolgus |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q0PW86 |
别名 | PDGF R α/PDGFRA Protein, Cynomolgus, Recombinant (His), RHEPDGFRA, PDGFRA, MGC74795, PDGFR2, CD140A, PDGFR-2 |
蛋白构建 | Gln24-Glu524 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 57.32 kDa (predicted). Due to glycosylation, the protein migrates to 70-85 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF. |
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PDGF R alpha/PDGFRA Protein, Cynomolgus, Recombinant (His) PDGF R α/PDGFRA Protein, Cynomolgus, Recombinant (His) MGC 74795 RHEPDGFRA MGC-74795 PDGFRA MGC74795 PDGFR 2 PDGFR2 CD140A PDGFR-2 recombinant recombinant-proteins proteins protein