PD-L1 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 35-40 KDa and the accession number is Q9NZQ7.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 500 | 5日内发货 | ||
50 μg | ¥ 1,170 | 5日内发货 | ||
500 μg | ¥ 8,860 | 5日内发货 | ||
1 mg | ¥ 12,600 | 5日内发货 |
生物活性 | 1. Loaded Human PD-1-Fc on Protein A Biosensor, can bind Human PD-L1-Flag with an affinity constant of 0.19 uM as determined in BLI assay. (Regularly tested) 2. Immobilized Human PD-L1-Flag at 2μg/ml (100 μl/well)can bind Anti-Human PDL1 mAb-Fc. The ED50 of Anti-Human PDL1 mAb-Fc is 14.68 ng/ml. (Regularly tested) |
产品描述 | PD-L1 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 35-40 KDa and the accession number is Q9NZQ7. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-Flag |
蛋白编号 | Q9NZQ7 |
别名 | B7-H1, Programmed Cell Death 1 Ligand 1, PD-L1, PDCD1L1, B7 Homolog 1, PDCD1LG1, PDL1, B7H1, CD274, PDCD1 Ligand 1, Programmed Death Ligand 1 |
氨基酸序列 | Phe19-Thr239 |
蛋白构建 | Phe19-Thr239 |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 35-40 KDa, reducing conditions |
内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 150 mM NaCl, pH 7.4. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers. |
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PD-L1 Protein, Human, Recombinant (Flag) PDL 1 B7-H1 CD 274 Programmed Cell Death 1 Ligand 1 PDL-1 PD-L1 PDCD1L1 B7 Homolog 1 PDCD1LG1 CD-274 PDL1 B7H1 CD274 PDCD1 Ligand 1 Programmed Death Ligand 1 recombinant recombinant-proteins proteins protein