PCSK9 Protein, Human, Recombinant (D374Y, His)

PCSK9 Protein, Human, Recombinant (D374Y, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 72.5 kDa and the accession number is Q8NBP7-1.

规格	价格/CNY		数量
50 μg	￥ 3,820	现货
100 μg	￥ 5,960	5日内发货
200 μg	￥ 9,300	5日内发货
500 μg	￥ 16,690	5日内发货

规格

价格/CNY

货期

数量

50 μg

￥ 3,820

现货

100 μg

￥ 5,960

5日内发货

200 μg

￥ 9,300

5日内发货

500 μg

￥ 16,690

5日内发货

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized Human LDLR mFc at 2 μg/ml (100 μl/well) can bind Human PCSK9(D374Y) His, the EC50 of PCSK9（D374Y) His is 1-20 ng/mL. 2.Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag on ProA Biosensor, can bind Recombinant Human PCSK9 (D374Y) Protein, His Tag with an affinity constant of 2.57nM as determined in BLI assay (Routinely tested).

TargetMol Bioactive

产品描述

PCSK9 Protein, Human, Recombinant (D374Y, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 72.5 kDa and the accession number is Q8NBP7-1.

种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q8NBP7-1
别名	LDLCQ1, NARC-1, PC9, NARC1, HCHOLA3, proprotein convertase subtilisin/kexin type 9, FH3
蛋白构建	The Human PCSK9 (NP_777596.2, with mutation Asp 374 Tyr) (Met1-Gln692) was expressed with a polyhistidine tag at the C-terminus.
蛋白纯度	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	72.5 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.