SARS-CoV-2 Papain-Like Protease Protein

Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.

规格	价格/CNY		数量
10 μg	￥ 1,170	5日内发货
50 μg	￥ 3,550	5日内发货
500 μg	￥ 18,900	5日内发货
1 mg	￥ 28,500	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 1,170

5日内发货

50 μg

￥ 3,550

5日内发货

500 μg

￥ 18,900

5日内发货

1 mg

￥ 28,500

5日内发货

产品描述

种属	SARS-CoV-2
表达系统	E. coli
标签	Tag Free
蛋白编号	QHD43415.1
别名	PLpro, PL-PRO, pp1a, Replicase polyprotein 1a, Papain-like Protease
氨基酸序列	Glu1564-Lys1878
蛋白构建	Glu1564-Lys1878
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	34 KDa (reducing condition)

缓冲液	Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 10 mM 2-Mercaptoethanol, 20% Glycerol, pH 7.5.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.

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SARS-CoV-2 Papain-Like Protease Protein

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