P-Selectin Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 98.7 kDa and the accession number is Q3MID1.
生物活性 | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to rat SELP/Fc Chimera coated plates (10 μg/mL with 100 μL/well), > 40% cells will adhere after 1 hour at 37℃. |
产品描述 | P-Selectin Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 98.7 kDa and the accession number is Q3MID1. |
种属 | Rat |
表达系统 | HEK293 Cells |
标签 | C-hFc |
蛋白编号 | Q3MID1 |
别名 | selectin P (granule membrane protein 140kDa, antigen CD62) |
蛋白构建 | The Rat Selp (AAI01920.1) (Met1-Gln700) was expressed with the Fc region of Human IgG1 at the C-terminus. |
蛋白纯度 | > 95 % as determined by SDS-PAGE. |
分子量 | 98.7 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. |
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P-Selectin Protein, Rat, Recombinant (hFc) granule membrane protein 140kDa, antigen CD62 selectin P selectin P (granule membrane protein 140kDa, antigen CD62) recombinant recombinant-proteins proteins protein