Nectin-4 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag. The predicted molecular weight is 40-50 KDa and the accession number is Q96NY8.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 2,260 | 5日内发货 | ||
100 μg | ¥ 6,130 | 5日内发货 |
生物活性 | 1. Loaded Biotinylated Human Nectin-4-His-Avi on SA Biosensor, can bind Anti-Human Nectin-4 mAb-mFc with an affinity constant of 1 <10-3 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human Nectin-4-His-Avi at 2μg/ml (100 μl/well) can bind Anti-Human Nectin-4 mAb-mFc. The ED50 of Anti-Human Nectin-4 mAb-mFc is 12.28 ng/ml. (Regularly tested) |
产品描述 | Nectin-4 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag. The predicted molecular weight is 40-50 KDa and the accession number is Q96NY8. |
种属 | Human |
表达系统 | Human Cells |
标签 | C-6His-Avi |
蛋白编号 | Q96NY8 |
别名 | Nectin-4, PVRL4, LNIR, PRR4, Ig superfamily receptor LNIR, Poliovirus receptor-related protein 4 |
氨基酸序列 | Gly32-Val351 |
蛋白构建 | Biotinylated Recombinant Human Poliovirus Receptor-Related Protein 4 is produced by our Mammalian expression system and the target gene encoding Gly32-Val351 is expressed with a 6His, Avi tag at the C-terminus. |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 40-50 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
复溶方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
存储 |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
运输方式 |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | Nectin-4 (PVRL4) is a type I transmembrane glycoprotein which belongs to the nectin family of Ig superfamily proteins. It contains two Ig-like C2-type domains and one Ig-like V-type domain. PVRL4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with nectin-1. It does not act as receptor for alpha-herpesvirus entry into cells. It is predominantly expressed in placenta, the embryo and breast carcinoma. But it is not detected in normal breast epithelium. The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. |
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Nectin-4 Protein, Human, Recombinant (His & Avi), Biotinylated Nectin-4 PVRL 4 PVRL4 LNIR PRR4 Ig superfamily receptor LNIR PRR-4 PRR 4 Poliovirus receptor-related protein 4 PVRL-4 recombinant recombinant-proteins proteins protein