KRAS Protein, Human, Recombinant (G12V, His)

KRAS Protein, Human, Recombinant (G12V, His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 25-30 KDa and the accession number is AAH13572.1.

规格	价格/CNY		数量
10 μg	￥ 320	5日内发货
50 μg	￥ 990	5日内发货
500 μg	￥ 5,030	5日内发货
1 mg	￥ 7,560	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 320

5日内发货

50 μg

￥ 990

5日内发货

500 μg

￥ 5,030

5日内发货

1 mg

￥ 7,560

5日内发货

产品描述	KRAS Protein, Human, Recombinant (G12V, His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 25-30 KDa and the accession number is AAH13572.1.

种属	Human
表达系统	E. coli
标签	N-6xHis
蛋白编号	AAH13572.1
别名	KRAS2, RASK2, c-K-ras, Ki-Ras, CFC2
氨基酸序列	Thr2-Cys185(Gly12Val)
蛋白构建	Thr2-Cys185(Gly12Val)
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	25-30 KDa (reducing condition)

内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	K-Ras belongs to the small GTPase superfamily, Ras family. As other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations have been found to be associated with Noonan syndrome[4] and cardio-facio-cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer and lung cancer.

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Desired Reconstitution Concentration

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KRAS Protein, Human, Recombinant (G12V, His)

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