IL-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag. The predicted molecular weight is 16-18 KDa and the accession number is P60568.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 2,260 | 5日内发货 | ||
100 μg | ¥ 6,130 | 5日内发货 |
生物活性 | 1. Loaded Human IL-2RA-Fc on Protein A Biosensor, can bind Biotinylated Human IL-2-His-Avi with an affinity constant of 8.60 nM as determined in BLI assay. (Regularly tested) 2. Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.2 ng/ml. (Regularly tested)3. Immobilized Human IL-2RA-His at 2μg/ml (100 μl/well)can bind Human IL-2-His-Avi. The ED50 of Human IL-2-His-Avi is 6.45 ng/ml. (Regularly tested) |
产品描述 | IL-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag. The predicted molecular weight is 16-18 KDa and the accession number is P60568. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-6xHis-Avi |
蛋白编号 | P60568 |
别名 | IL2, IL-2, 白介素, Interleukin-2, Aldesleukin, T-Cell Growth Factor, TCGF, 白细胞介素 |
氨基酸序列 | Ala21-Thr153 |
蛋白构建 | Ala21-Thr153 |
蛋白纯度 | Greater than 90% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 16-18 KDa (reducing condition) |
内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Interleukin-2(IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system,belongs to the IL-2 family. It is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. |
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IL-2 Protein, Human, Recombinant (His & Avi), Biotinylated IL 2 IL2 IL-2 白介素 Interleukin-2 Aldesleukin T-Cell Growth Factor TCGF 白细胞介素 recombinant recombinant-proteins proteins protein