IL-17F Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 19 KDa and the accession number is AAH70124.1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 820 | 5日内发货 | ||
50 μg | ¥ 2,480 | 5日内发货 | ||
500 μg | ¥ 12,100 | 5日内发货 | ||
1 mg | ¥ 17,400 | 5日内发货 |
生物活性 | 1. Loaded Biotinylated Human IL-17RA-His-Avi on SA Biosensor, can bind Human IL-17F -His with an affinity constant of 81.3 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Mouse IL-17RA-Fc at 1μg/ml (100 μl/well)can bind Human IL-17F-His. The ED50 of Human IL-17F-His is 47.94 ng/ml. (Regularly tested) |
产品描述 | IL-17F Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 19 KDa and the accession number is AAH70124.1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-6xHis |
蛋白编号 | AAH70124.1 |
别名 | IL17F, IL-17F, Cytokine ML-1, Interleukin-17F |
氨基酸序列 | Arg31-Gln163 |
蛋白构建 | Arg31-Gln163 |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 19 KDa (reducing condition) |
内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 150 mM NaCl, pH 7.4. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Interleukin-17F (IL-17F) exists in a disulfide-linked heterodimer that belongs to the IL-17 family. IL-17F is expressed in activated, but not resting, CD4+ T-cells and activated monocytes. IL-17F has been shown to stimulate the production of several other cytokines, including IL-6, IL-8, and granulocyte colony-stimulating factor. IL-17F can regulate cartilage matrix turnover and stimulates PBMC and T-cell proliferation. IL-17F is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Defects in IL-17F are the cause of familial candidiasis type 6 (CANDF6). |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
IL-17F Protein, Human, Recombinant (His) IL17F IL-17F Cytokine ML-1 Interleukin-17F recombinant recombinant-proteins proteins protein