IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. IFN-alpha 1/IFNA1 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 45.88 kDa and the accession number is P01572.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | ||
500 μg | ¥ 12,400 | 5日内发货 | ||
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 | Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IFN alpha 1, hFc Tag with an affinity constant of 11.96 nM as determined in SPR assay. |
产品描述 | IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. IFN-alpha 1/IFNA1 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 45.88 kDa and the accession number is P01572. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-hFc |
蛋白编号 | P01572 |
别名 | IFN-α-1/13, IFN alpha1, 干扰素, Interferon α-1/13, Interferon alpha-1/13, IFNalpha 1, Interferon α-D, IFNA1, IFNα1, IFN-alpha-1, IFN α1, LeIF D, IFN α 1, IFN-α-1, Interferon α1, IFN-alpha-1/13, Interferon alpha 1, Interferon alpha-D |
蛋白构建 | Cys24-Lys189 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | 45.88 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. |
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IFN-alpha 1/IFNA1 Protein, Mouse, Recombinant (hFc) IFNA 1 IFN-α-1/13 IFN-a-1 IFN alpha1 干扰素 Interferon α-1/13 Interferon alpha-1/13 IFNalpha 1 Interferon α-D Interferon a-D IFNA1 Interferon a 1 IFNα1 IFN-alpha-1 IFN α1 LeIF D IFN-a-1/13 Interferon a-1/13 IFN α 1 Interferon α 1 IFN-α-1 IFNα 1 Interferon α1 IFNA-1 IFN-alpha-1/13 Interferon alpha 1 Interferon alpha-D recombinant recombinant-proteins proteins protein