In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. GM-CSFR alpha Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 37.7 kDa and the accession number is P15509-1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 5,180 | 5日内发货 | ||
500 μg | ¥ 20,700 | 5日内发货 | ||
1 mg | ¥ 34,600 | 5日内发货 |
生物活性 | 1. Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 7.7ng/ml determined by ELISA. 2. Biotinylated Human GM-CSF R alpha, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay. |
产品描述 | In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. GM-CSFR alpha Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 37.7 kDa and the accession number is P15509-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | P15509-1 |
别名 | GM-CSF-R-α, GMR-α, GMR-alpha, GM-CSF-R-alpha, CSF2R, GMCSFR-alpha, CSF2RAY, GMCSFR-α, GMR, CSF2RAX, CSF2RX, CDw116, SMDP4, GM-CSF R α Protein, CSF2RY, 集落刺激因子, GMCSFR, CSF2RA, CD116 |
蛋白构建 | Glu23-Gly320 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 37.7 kDa (predicted). Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. |
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GM-CSFR alpha Protein, Human, Recombinant (His & Avi), Biotinylated GM-CSF-R-α GMR-α CD-116 GMR-alpha GM-CSF-R-alpha CSF2R GMCSFR-alpha CSF2RAY GMCSFR-α GMR CSF2RAX CSF2RX SMDP-4 SMDP 4 CDw116 SMDP4 CD 116 GM-CSF R α Protein CSF2RY 集落刺激因子 GMCSFR CSF2RA CD116 recombinant recombinant-proteins proteins protein