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Ephrin B2/EFNB2 Protein, Human, Recombinant (His & hFc)

产品编号 TMPY-01287

别名: Htk-L, ephrin-B2, EPLG5, HTKL, LERK5

Ephrin B2/EFNB2 Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 50.3 kDa and the accession number is P52799.

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Ephrin B2/EFNB2 Protein, Human, Recombinant (His & hFc)

规格	价格/CNY		货期	数量
100 μg	￥ 2,940		5日内发货

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参考文献

生物活性	Measured by its binding ability in a functional ELISA. Immobilized human EphB4-his at 2 μg/ml (100 μl/well) can bind human EphrinB2 Fc chimera with a linear range of 1-25 ng/ml. Immobilized human EphB4 at 2 μg/ml (100 μl/well) can bind human EphrinB2 Fc chimera with a linear range of 1-125 ng/ml.
产品描述	Ephrin B2/EFNB2 Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 50.3 kDa and the accession number is P52799.

种属	Human
表达系统	HEK293 Cells
标签	C-hFc-His
蛋白编号	P52799
别名	Htk-L, ephrin-B2, EPLG5, HTKL, LERK5
蛋白构建	The Human EFNB2 (NP_004084.1) extracellular domain (Met 1-Ala 229) was was fused with a C-terminal Fc region of Human IgG1 tag followed by a polyhistidine tag.
蛋白纯度	> 97 % as determined by SDS-PAGE
分子量	50.3 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	EphrinB2 also known as EFNB2 is a member of the ephrin family. EphrinB2 is involved in establishing arterial versus venous identity and perhaps in anastamosing arterial and venous vessels at their junctions. The transmembrane-associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB2 expression progressively extends from the arterial endothelium to surrounding smooth muscle cells and to pericytes, suggesting that ephrin-B2 may play an important role during formation of the arterial muscle wall.

参考文献

1. Wang HU,et al.(1998) Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4. Cell. 93(5): 741-53. 2. Gale NW,et al.(2001) Ephrin-B2 selectively marks arterial vessels and neovascularization sites in the adult, with expression in both endothelial and smooth-muscle cells. Dev Biol. 230(2): 151-60. 3. Shin D,et al.(2001) Expression of ephrinB2 identifies a stable genetic difference between arterial and venous vascular smooth muscle as well as endothelial cells, and marks subsets of microvessels at sites of adult neovascularization. Dev Biol. 230(2): 139-50.

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Keywords

Ephrin B2/EFNB2 Protein, Human, Recombinant (His & hFc) Htk-L ephrin-B2 EPLG 5 EPLG5 LERK 5 HTKL LERK-5 LERK5 EPLG-5 recombinant recombinant-proteins proteins protein

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