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Ephrin B1/EFNB1 Protein, Human, Recombinant (His & hFc)

产品编号 TMPY-01286

别名: EFL3, Elk-L, CFNS, LERK2, EFB1, EPLG2, ephrin-B1, CFND

Ephrin B1/EFNB1 Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 51.2 kDa and the accession number is P98172.

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Ephrin B1/EFNB1 Protein, Human, Recombinant (His & hFc)

规格	价格/CNY		货期	数量
100 μg	￥ 2,940		5日内发货

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参考文献

生物活性	Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2 μg/ml (100 μl/well) can bind human EFNB1 Fc chimera with a linear range of 1.56-25 ng/ml.
产品描述	Ephrin B1/EFNB1 Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 51.2 kDa and the accession number is P98172.

种属	Human
表达系统	HEK293 Cells
标签	C-hFc-His
蛋白编号	P98172
别名	EFL3, Elk-L, CFNS, LERK2, EFB1, EPLG2, ephrin-B1, CFND
蛋白构建	The Human EFNB1 (NP_004420.1) extracellular domain (Met 1-Lys 237) was was fused with a C-terminal Fc region of Human IgG1 tag followed by a polyhistidine tag.
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	51.2 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

参考文献

1. Davy A, et al. (2004) Ephrin-B1 forward and reverse signaling are required during mouse development. Genes Dev. 18(5): 572-83. 2. Compagni A, et al. (2003) Control of skeletal patterning by ephrinB1-EphB interactions. Dev Cell. 5(2): 217-30. 3. Wieland I, et al. (2004) Mutations of the ephrin-B1 gene cause craniofrontonasal syndrome. Am J Hum Genet. 74(6): 1209-15.

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Keywords

Ephrin B1/EFNB1 Protein, Human, Recombinant (His & hFc) EFL-3 EFL3 Elk-L EPLG 2 EFB 1 CFNS LERK2 EFB1 EFB-1 EPLG2 LERK-2 LERK 2 EPLG-2 EFL 3 ephrin-B1 CFND recombinant recombinant-proteins proteins protein

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