Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 28.2 kDa and the accession number is P16422.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 1,920 | 5日内发货 | ||
500 μg | ¥ 7,710 | 5日内发货 | ||
1 mg | ¥ 12,800 | 5日内发货 |
生物活性 | Immobilized Human EpCAM, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. |
产品描述 | Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 28.2 kDa and the accession number is P16422. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | P16422 |
别名 | DIAR5, MOC31, EGP, MK1, HNPCC8, EGP314, ESA, MIC18, TROP1, EGP40, EpCAM, TACSTD1, gp40, CD326, TACST-1, EGP-2, ACSTD1, KSA |
蛋白构建 | Gln24-Lys265 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 28.2 kDa (predicted). Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His) DIAR5 MIC-18 MOC31 HNPCC 8 MOC 31 ACSTD 1 HNPCC-8 EGP2 MOC-31 EGP-40 EGP MK1 CD 326 TROP 1 MK 1 HNPCC8 EGP314 ESA MIC18 TROP-1 EGP 314 TROP1 EGP40 EpCAM EGP 40 MK-1 EGP 2 TACST 1 ACSTD-1 DIAR-5 EGP-314 TACSTD1 TACSTD 1 gp40 CD326 MIC 18 TACST1 TACST-1 EGP-2 ACSTD1 KSA CD-326 TACSTD-1 DIAR 5 recombinant recombinant-proteins proteins protein