EGFR Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 95 kDa and the accession number is P00533-1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,090 | 5日内发货 | ||
1 mg | ¥ 13,700 | 5日内发货 |
生物活性 | Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGF at 10 μg/ml (100 μl/well) can bind human EGFR with a linear range of 0.64-400 ng/ml. |
产品描述 | EGFR Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 95 kDa and the accession number is P00533-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-hFc |
蛋白编号 | P00533-1 |
别名 | ERBB, mENA, HER1, ERBB1, PIG61, NISBD2, epidermal growth factor receptor |
蛋白构建 | The extracellular domain (Met 1-Ser 645) of Human EGFR (NP_005219) was fused with the Fc region of Human IgG1 at the C-terminus. |
蛋白纯度 | > 97 % as determined by SDS-PAGE. >85% as determined by SEC-HPLC |
分子量 | 95 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
EGFR Protein, Human, Recombinant (hFc) ERBB PIG 61 mENA NISBD-2 HER1 ERBB1 ERBB-1 PIG-61 PIG61 NISBD2 NISBD 2 epidermal growth factor receptor HER 1 ERBB 1 HER-1 recombinant recombinant-proteins proteins protein