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EGFL7 Protein, Mouse, Recombinant (His)

产品编号 TMPY-02178

别名: Zneu1, VE-statin, EGF-like-domain, multiple 7

EGFL7 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 29.8 kDa and the accession number is Q9QXT5-1.

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EGFL7 Protein, Mouse, Recombinant (His)

规格	价格/CNY		货期	数量
100 μg	￥ 3,930		5日内发货

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生物活性	Testing in progress
产品描述	EGFL7 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 29.8 kDa and the accession number is Q9QXT5-1.

种属	Mouse
表达系统	Baculovirus Insect Cells
标签	N-His
蛋白编号	Q9QXT5-1
别名	Zneu1, VE-statin, EGF-like-domain, multiple 7
蛋白构建	The Mouse EGFL7 isoform 1 (Q9QXT5-1) (Glu 22-Leu 275) was fused with a polyhistidine tag at the N-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	29.8 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 10% gly, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Epidermal growth factor-like protein 7, also known as EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, Multiple EGF-like domains protein 7, Vascular endothelial statin, NOTCH4-like protein and EGFL7, is a secreted protein which contains twoEGF-like domains and oneEMI domain. EGFL7 was identified by a number of groups as a putative secreted factor produced by the vascular endothelial cells (ECs). EGFL7 is described as a novel endothelial cell-derived factor involved in the regulation of the spatial arrangement of cells during vascular tube assembly. With its impact on tubulogenesis and vessel shape EGFL7 joined the large family of molecules governing blood vessel formation. EGFL7 regulates midline angioblast migration in zebrafish embryos-a key step in vascular tubulogenesis. EGFL7 is tightly associated with the extracellular matrix (ECM), and it supports EC migration either as a single factor or in combination with other ECM molecules. EGFL7 provides a proper microenvironment for endothelial cell migration, thereby enabling accurate patterning. Our study indicates that the molecular composition of the ECM influences vascular morphogenesis. EGFL7 also regulates vascular tubulogenesis. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate.

参考文献

1. Fitch,M.J. et al., 2004, Dev Dyn. 230 (2):316-24. 2. Schmidt,M. et al., 2007, Novartis Found Symp. 283 :18-28. 3. Campagnolo,L.et al., 2008, Gene Expr Patterns 8 (6):389-96. 4. Picuric,S. et al., 2009, Protein Expr Purif. 68 (1):1-6. 5. Nikolic,I. et al., 2010, J Angiogenes Res. 2 (1): 9.

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Keywords

EGFL7 Protein, Mouse, Recombinant (His) Zneu1 VE-statin EGF-like-domain, multiple 7 recombinant recombinant-proteins proteins protein

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