CD47 Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 mammalian cells with C-Avi-6xHis tag. The predicted molecular weight is 30-40 KDa and the accession number is Q08722.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 2,260 | 5日内发货 | ||
100 μg | ¥ 6,130 | 5日内发货 |
生物活性 | Loaded Anti-Human CD47 mAb-Fc on Protein A Biosensor, can bind Human CD47-Avi-6His with an affinity constant of 0.20 nM as determined in BLI assay. (Regularly tested) |
产品描述 | CD47 Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 mammalian cells with C-Avi-6xHis tag. The predicted molecular weight is 30-40 KDa and the accession number is Q08722. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-Avi-6xHis |
蛋白编号 | Q08722 |
别名 | Protein MER6, Integrin-Associated Protein, MER6, CD47, Leukocyte Surface Antigen CD47, Antigenic Surface Determinant Protein OA3, IAP |
氨基酸序列 | Gln19-Pro139 |
蛋白构建 | Gln19-Pro139 |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 30-40 KDa, reducing conditions |
内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 10 mMTris-Citrate, 150 mM NaCl, pH 8.0. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | CD47(Integrin-Associated Protein,IAP) is a 40 ‑ 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
CD47 Protein, Human, Recombinant (Avi & His), Biotinylated Protein MER6 CD 47 Integrin-Associated Protein MER6 CD-47 MER 6 CD47 Leukocyte Surface Antigen CD47 MER-6 Antigenic Surface Determinant Protein OA3 IAP recombinant recombinant-proteins proteins protein