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Carbonic Anhydrase 4 Protein, Human, Recombinant (His)

产品编号 TMPY-00513

别名: CAIV, Car4, RP17, carbonic anhydrase IV

Carbonic Anhydrase 4 Protein, Human, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 31.7 kDa and the accession number is P22748-1.

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Carbonic Anhydrase 4 Protein, Human, Recombinant (His)

规格	价格/CNY		货期	数量
20 μg	￥ 2,270		5日内发货

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参考文献

生物活性	Measured by its esterase activity. The specific activity is >10 pmol/min/μg.
产品描述	Carbonic Anhydrase 4 Protein, Human, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 31.7 kDa and the accession number is P22748-1.

种属	Human
表达系统	CHO Cells
标签	C-His
蛋白编号	P22748-1
别名	CAIV, Car4, RP17, carbonic anhydrase IV
蛋白构建	The Human CA4 (NP_000708.1) (Met1-Lys283) without the pro peptide was expressed with a polyhistidine tag at the C-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	31.7 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3-dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

参考文献

1. Lehtonen J,et al.(2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27. 2. Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1)：1-20. 3. Baird TT,et al.(1997) Catalysis and Inhibition of Human Carbonic Anhydrase IV. Biochemistry. 36 (9): 2669-78.

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Keywords

Carbonic Anhydrase 4 Protein, Human, Recombinant (His) CAIV Car4 RP17 RP 17 carbonic anhydrase IV RP-17 recombinant recombinant-proteins proteins protein

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