BTLA Protein, Rat, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 43.4 kDa and the accession number is Q6PNM1-1.
生物活性 | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized rat BTLA-mFc at 10 μg/mL (100 μL/well) can bind mouse HVEM-Fch . The EC50 of mouse EFNA5-Fc is 6.2-14.4 ng/mL. |
产品描述 | BTLA Protein, Rat, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 43.4 kDa and the accession number is Q6PNM1-1. |
种属 | Rat |
表达系统 | HEK293 Cells |
标签 | C-mFc |
蛋白编号 | Q6PNM1-1 |
别名 | B and T lymphocyte associated |
蛋白构建 | The Rat Btla (NP_998795.1) (Ala6-Tyr183) was expressed with the Fc region of Mouse IgG1 at the C-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE. |
分子量 | 43.4 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | BTLA is a inhibitory molecule which belongs to the Ig superfamily. It down-modulates immune responses. As such, reagents that regulate the binding of BTLA to its ligand or alter BTLA signaling have significant therapeutic promise. BTLA is crucial to understand the mechanism(s) of action of these antibodies before attempting clinical applications. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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BTLA Protein, Rat, Recombinant (mFc) B and T lymphocyte associated recombinant recombinant-proteins proteins protein