BMP-4 Protein, Human, Recombinant (E399D) is expressed in E. coli expression system. The predicted molecular weight is 13.25 kDa and the accession number is P12644.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 1,060 | 现货 | ||
100 μg | ¥ 4,430 | 5日内发货 | ||
200 μg | ¥ 7,790 | 5日内发货 |
生物活性 |
Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 0.08-0.4ug/mL.
|
产品描述 | BMP-4 Protein, Human, Recombinant (E399D) is expressed in E. coli expression system. The predicted molecular weight is 13.25 kDa and the accession number is P12644. |
种属 | Human |
表达系统 | E. coli |
标签 | Tag Free |
蛋白编号 | P12644 |
别名 | OFC11, BMP2B, bone morphogenetic protein 4, BMP2B1, ZYME, MCOPS6 |
蛋白构建 | Human BMP4 (P12644) (Ser293-Arg408, E399D) |
蛋白纯度 |
≥ 95 % as determined by SDS-PAGE.
|
分子量 | 13.25 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 30% Acetonitrile, 1/1000 TFA. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | BMP4 (bone morphogenetic protein-4)-directed differentiation of human embryonic stem cells (ESCd). Autophagy is a conserved catabolic process with complicated roles in tumor development. Bone morphogenetic protein 4 (BMP4), a member of the transforming growth factor (TGF-β) family of regulatory proteins, plays a crucial role in human malignancies. BMP4 treatment promoted HCC cells proliferation and induced autophagy. Mechanistic study revealed that the induction of autophagy by BMP4 was mediated through activating the JNK1/Bcl2 pathway. And the JNK1 inhibitor and knockdown of JNK1 could attenuate autophagy induced by BMP4 and eliminated BMP4-promoted HCC cells growth. BMP4 promoted HCC proliferation by autophagy activation through JNK1/Bcl-2 signaling. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
BMP-4 Protein, Human, Recombinant (E399D) OFC11 OFC 11 MCOPS 6 OFC-11 MCOPS-6 BMP2B bone morphogenetic protein 4 BMP2B1 ZYME MCOPS6 recombinant recombinant-proteins proteins protein