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alanyl-tRNA synthetase Protein, Mouse, Recombinant (His)

alanyl-tRNA synthetase Protein, Mouse, Recombinant (His)

产品编号 TMPY-02441
别名: sti, C76919, AI316495, alanyl-tRNA synthetase

alanyl-tRNA synthetase Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 108.3 kDa and the accession number is Q8BGQ7.

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alanyl-tRNA synthetase Protein, Mouse, Recombinant (His)
规格 价格/CNY 货期 数量
100 μg ¥ 3,820 5日内发货
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产品目录号及名称: alanyl-tRNA synthetase Protein, Mouse, Recombinant (His) (TMPY-02441)
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生物活性 Testing in progress
产品描述 alanyl-tRNA synthetase Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 108.3 kDa and the accession number is Q8BGQ7.
种属 Mouse
表达系统 Baculovirus Insect Cells
标签 C-His
蛋白编号 Q8BGQ7
别名 sti, C76919, AI316495, alanyl-tRNA synthetase
蛋白构建 The Mouse AARS (Q8BGQ7) (Met 1-Asn 968) was fused with a polyhistidine tag at the C-terminus.
蛋白纯度 > 88 % as determined by SDS-PAGE
分子量 108.3 kDa (predicted)
内毒素 < 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice.

研究背景 Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections.

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Keywords

alanyl-tRNA synthetase Protein, Mouse, Recombinant (His) sti AI 316495 C76919 C 76919 C-76919 AI-316495 AI316495 alanyl-tRNA synthetase recombinant recombinant-proteins proteins protein

 

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