15 Lipoxygenase 2 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 76 kDa and the accession number is AAH35217.1.
生物活性 | Testing in progress |
产品描述 | 15 Lipoxygenase 2 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 76 kDa and the accession number is AAH35217.1. |
种属 | Human |
表达系统 | Baculovirus Insect Cells |
标签 | Tag Free |
蛋白编号 | AAH35217.1 |
别名 | arachidonate 15-lipoxygenase, type B, ALOX15B, 15-LOX-2 |
蛋白构建 | The Human ALOX15B (AAH35217.1)( Met1-Ile676) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 76 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. ALOX15B converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. ALOX15B gene is located in a cluster of related genes and a pseudogene that spans approximately 1 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. |
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15 Lipoxygenase 2 Protein, Human, Recombinant arachidonate 15-lipoxygenase, type B ALOX15B 15-LOX-2 recombinant recombinant-proteins proteins protein