Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Belotecan hydrochloride (CKD-602) 是一种合成的水溶性喜树碱衍生物和拓扑异构酶 I 抑制剂,具有潜在的抗肿瘤活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 413 | 现货 | ||
5 mg | ¥ 980 | 现货 | ||
10 mg | ¥ 1,490 | 现货 | ||
25 mg | ¥ 2,230 | 现货 | ||
50 mg | ¥ 2,980 | 现货 | ||
100 mg | ¥ 3,890 | 现货 | ||
500 mg | ¥ 8,290 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 972 | 现货 |
产品描述 | Belotecan hydrochloride (CKD-602) is a synthetic water-soluble camptothecin derivative and topoisomerase I inhibitor with potential antitumor activity |
体外活性 | Belotecan hydrochloride(CKD-602) was revealed to exert a significant cytotoxic effect on all cell lines in a time- and dose-dependent manner.?The cell viability IC50 values were 2.4 μg/ml for YD-8, 0.18 μg/ml for YD-9 and 0.05 μg/ml for YD-38 cells at 72 h following treatment[1]. |
细胞实验 | Cells at a density of 2×10^4 cells/well in 100 μl RPMI with 10% FBS were added to the wells of a 96-well plate.?The cells were treated with different concentrations (0.01, 0.1, 0.5, 1, 5 and 10 μg/ml) of CKD-602 for 24, 48 and 72 h. Control samples of each cell line were treated with medium only.?For the viability assay, 20 μl/well CellTiter 96AQueous One Solution Reagent (MTS) was added.?After 1 h incubation at 37°C in a humidified atmosphere of 5% CO2, the absorbance at 490 nm was recorded using an ELISA plate reader ?The assay was performed in triplicate with three independent experiments for each condition.?The data from the treatment groups were normalized to those of the control samples and are presented as the mean ±standard error of the mean.?The half maximal (50%) inhibitory concentration (IC50) values were calculated from the dose-response curve[1]. |
别名 | CKD-602 |
分子量 | 469.96 |
分子式 | C25H28ClN3O4 |
CAS No. | 213819-48-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (106.39 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.1278 mL | 10.6392 mL | 21.2784 mL | 53.196 mL |
5 mM | 0.4256 mL | 2.1278 mL | 4.2557 mL | 10.6392 mL | |
10 mM | 0.2128 mL | 1.0639 mL | 2.1278 mL | 5.3196 mL | |
20 mM | 0.1064 mL | 0.532 mL | 1.0639 mL | 2.6598 mL | |
50 mM | 0.0426 mL | 0.2128 mL | 0.4256 mL | 1.0639 mL | |
100 mM | 0.0213 mL | 0.1064 mL | 0.2128 mL | 0.532 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
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